Articles
CRYOPRESERVATION OF IN VITRO APICES OF STRAWBERRY (FRAGARIA X ANANASSA DUCH.) BY A NEW ENCAPSULATION-DEHYDRATION METHOD
Article number
649_21
Pages
115 – 118
Language
English
Abstract
Cryopreservation of shoots apices of strawberry by encapsulation-dehydration method has been successfully carried out in some cultivars (Clavero-Ramírez et al., 2001). However, other cultivars have produced low viability with the same method.
A new protocol of encapsulation-dehydration (Sakai et al., 2000) has been used to improve shoot formation rate of these cultivars.
In this new protocol, the apices are encapsulated in a medium with glycerol and the pre-treatment with high sucrose concentration, required by conventional procedure, is eliminated.
We present the preliminary studies carried out using this new method with several strawberry cultivars (Andana, Camarosa, Carisma) and a wild genotype (F. virginiana).
A new protocol of encapsulation-dehydration (Sakai et al., 2000) has been used to improve shoot formation rate of these cultivars.
In this new protocol, the apices are encapsulated in a medium with glycerol and the pre-treatment with high sucrose concentration, required by conventional procedure, is eliminated.
We present the preliminary studies carried out using this new method with several strawberry cultivars (Andana, Camarosa, Carisma) and a wild genotype (F. virginiana).
Authors
C. Ramírez, J.M. López-Aranda, J. Gálvez, C. Soria, J.F. Sánchez-Sevilla, J.J. Medina, M.E. Gónzalez-Benito
Keywords
meristems, long-term conservation, cold-hardening, germplasm, liquid nitrogen, silica gel.
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