Articles
CHARACTERIZATION OF PORTUGUESE GRAPEVINE CULTIVARS USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS
Article number
652_53
Pages
401 – 405
Language
English
Abstract
Random amplified polymorphic DNA (RAPD) markers were used to characterize 24 Portuguese Vitis vinifera cultivars, representative of the Douro and Vinhos Verdes regions.
The eight primers used to study Vitis cultivars provided 49 reliable bands, of which 45 (91,8%) were polymorphic.
The UPGMA dendrogram obtained using the 49 bands scored showed that all the studied cultivars can be distinguished at a similarity level between 0.62 and 0.90. Alvarinho and Malvasia Fina present a great similarity, Tinta Francisca and Tinto Cão, Amaral and Touriga Franca, Tinta Barroca and Touriga Nacional, Moscatel Galego and Negra Mole are also close.
Jaen is the cultivar most distant from the others.
The eight primers used to study Vitis cultivars provided 49 reliable bands, of which 45 (91,8%) were polymorphic.
The UPGMA dendrogram obtained using the 49 bands scored showed that all the studied cultivars can be distinguished at a similarity level between 0.62 and 0.90. Alvarinho and Malvasia Fina present a great similarity, Tinta Francisca and Tinto Cão, Amaral and Touriga Franca, Tinta Barroca and Touriga Nacional, Moscatel Galego and Negra Mole are also close.
Jaen is the cultivar most distant from the others.
Authors
F. Leal, I. Castro, H. Guedes-Pinto, O. Pinto-Carnide, J.M. Ortiz, J.P. Martãn
Keywords
Vitis vinifera L., genomic analysis, PCR, RAPD
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