Home > I International Symposium on Grapevine Growing, Commerce and Research > OPTIMIZATION OF A PARTICLE BOMBARDMENT PROTOCOL FOR VITIS VINIFERA CV. ‘TRINCADEIRA’ AND CV. ‘ARAGONEZ’ TRANSFORMATION

Articles

OPTIMIZATION OF A PARTICLE BOMBARDMENT PROTOCOL FOR VITIS VINIFERA CV. ‘TRINCADEIRA’ AND CV. ‘ARAGONEZ’ TRANSFORMATION

Article number

652_54

Pages

407 – 413

Language

English

Abstract

Embryogenic calli derived from ovaries and stamens of two Vitis vinifera L. cultivars, ‘Trincadeira’ and ‘Aragonez’ were bombarded with different shot pressures using gold particles coated with WRG 1515 plasmid encoding for the beta

-glucuronidase (GUS) and hygromycin (HPTII) genes.
Two days after bombardment, the GUS activity, evaluated by histochemical analysis, revealed 1350 and 1550 psi as the best pressures.
Bombarded calli showed a strong sensitivity to the hygromycin concentrations tested.
Embryos formation only occurred on medium devoid of hygromycin, two months after bombardment.
These embryos were then transferred to the selection medium (0.4 mg/l of hygromycin).

Publication

I International Symposium on Grapevine Growing, Commerce and Research

Authors

H.G. Cardoso, A. Peixe, A. Baldé, M.S. Pais

Keywords

GUS, hygromycin, fungal diseases, somatic embryogenesis

Full text

https://doi.org/10.17660/ActaHortic.2004.652.54

Online Articles (70)

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