Articles
REGENERATION OF VIRUS-FREE PLANTS BY IN VITRO CHEMOTHERAPY OF GFLV (GRAPEVINE FANLEAF VIRUS) INFECTED EXPLANTS OF VITIS VINIFERA L. CV ‘ZALEMA’
Article number
652_61
Pages
463 – 466
Language
English
Abstract
In vitro chemotherapy using ribavirin or acyclovir was applied to eliminate Grapevine fanleaf virus (GFLV) from Vitis vinifera cv ‘Zalema’ plant material.
Ribavirin at 20 mgl-1 of concentration eliminated GFLV from the 94% of the grapevine in vitro shoots, enabling GFLV virus free plants to be obtained.
The ribavirin treatment did not affect plant growth, rooting and number of roots, and did not cause chlorosis, hyperhydration or apex necrosis.
Acyclovir at the same concentration was ineffective in regenerating GFLV-free plants and clearly negatively affected the explant development producing apical necrosis.
Ribavirin at 20 mgl-1 of concentration eliminated GFLV from the 94% of the grapevine in vitro shoots, enabling GFLV virus free plants to be obtained.
The ribavirin treatment did not affect plant growth, rooting and number of roots, and did not cause chlorosis, hyperhydration or apex necrosis.
Acyclovir at the same concentration was ineffective in regenerating GFLV-free plants and clearly negatively affected the explant development producing apical necrosis.
Authors
C.M. Weiland, M. Cantos, A. Troncoso, F. Perez-Camacho
Keywords
acyclovir, ribavirin, Vitis vinifera
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