Articles
THE EFFECT OF ION EXCHANGE SUBSTRATE AND SUCCINIC ACID ON EX VITRO ADAPTATION OF THE CHERRY ROOTSTOCK ‘VSL-2’ (PRUNUS FRUTICOSA PALL. × P. LANNESIANA CARR.)
Article number
795_60
Pages
401 – 408
Language
English
Abstract
Ex vitro adaptation is a period of stress for plants after in vitro culture.
Our research on the rootstock VSL-2 (Prunus fruticosa Pall. × P. Lannesiana Carr.) found a significant influence and lingering effect of soil (peat:sand, 3:1), BIONA-312, BIONA-312 + 1 mg/L succinic acid (SA), BIONA-312 + 10 mg/L SA, and BIONA-312 + 100 mg/L SA on adaptation rate, root length and number, stem length, chlorophyll (Chl) (a+b) content, sugar concentration (glucose + fructose + saccharose) and total amount of phenols in the plant leaves after a 16-week period of the 1st adaptation stage, carried out in adaptation substrates and the 2nd adaptation stage, carried out in soil.
The ion exchange substrate (IES) promoted a higher adaptation rate and better morphophysiological plant development.
We established the positive effect of a low SA concentration on ex vitro adaptation but a high concentration decreased plant number.
By using IES and SA during adaptation, we found that the sugar concentration in plant leaves decreased.
Also, the phenol concentration increased when using IES either with or without SA added.
The use of IES decreased Chl (a+b) content; however, addition of SA to IES gave different reactions depending on SA concentration.
At the 2nd adaptation stage, the plants adapted in IES and IES+SA had longer stems and higher dry weights and Chl content.
At this stage, the plants adapted in different substrates did not differ in sugar concentration but a high SA concentration in IES at the 1st stage stimulated increases in sugar and phenol concentration during further development.
The positive influence of IES and a low SA concentration on morphophysiological and biochemical parameters of plants during adaptation were established.
This effect remained during further plant cultivation on traditional substrates.
Our research on the rootstock VSL-2 (Prunus fruticosa Pall. × P. Lannesiana Carr.) found a significant influence and lingering effect of soil (peat:sand, 3:1), BIONA-312, BIONA-312 + 1 mg/L succinic acid (SA), BIONA-312 + 10 mg/L SA, and BIONA-312 + 100 mg/L SA on adaptation rate, root length and number, stem length, chlorophyll (Chl) (a+b) content, sugar concentration (glucose + fructose + saccharose) and total amount of phenols in the plant leaves after a 16-week period of the 1st adaptation stage, carried out in adaptation substrates and the 2nd adaptation stage, carried out in soil.
The ion exchange substrate (IES) promoted a higher adaptation rate and better morphophysiological plant development.
We established the positive effect of a low SA concentration on ex vitro adaptation but a high concentration decreased plant number.
By using IES and SA during adaptation, we found that the sugar concentration in plant leaves decreased.
Also, the phenol concentration increased when using IES either with or without SA added.
The use of IES decreased Chl (a+b) content; however, addition of SA to IES gave different reactions depending on SA concentration.
At the 2nd adaptation stage, the plants adapted in IES and IES+SA had longer stems and higher dry weights and Chl content.
At this stage, the plants adapted in different substrates did not differ in sugar concentration but a high SA concentration in IES at the 1st stage stimulated increases in sugar and phenol concentration during further development.
The positive influence of IES and a low SA concentration on morphophysiological and biochemical parameters of plants during adaptation were established.
This effect remained during further plant cultivation on traditional substrates.
Publication
Authors
T. Krasinskaya, N. Koukhartchik, V. Matushevich
Keywords
BIONA-312, chlorophylls, phenols, sugar
Online Articles (157)