Articles
INVESTIGATION OF ISOZYME POLYMORPHISM IN OPEN-POLLINATED SWEET CHERRY AND ‘MAHALEB’ SEEDLINGS
Article number
795_64
Pages
423 – 428
Language
English
Abstract
Traditionally, sweet cherry (Prunus avium L.) cultivars are propagated on seedling rootstocks in Turkey.
For this purpose, wild cherries (P. avium) and Mahaleb (P. mahaleb L.) have been used extensively.
The use of clonal (vegetatively propagated) rootstocks for sweet cherries is low in Turkey due to the high cost of nursery plants.
Seeds for rootstock propagation usually are collected by nurserymen from trees of unknown origin.
The objectives of this study were to compare the seedlings of two Prunus species for morphological characteristics and to detect any isozyme polymorphisms in seedling populations.
During the study, variations in germination rate, leaf area and seedling size were evaluated.
Isozyme variation was surveyed for five enzymes including alcohol dehydrogenase (ADH, EC 1.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.11.1.37), peroxidase (PRX, EC 1.11.1.7) and phosphoglucomutase (PGM, EC 2.7.5.1) in the populations.
Wild cherry (P. avium) seedlings exhibited greater variation than P. mahaleb. Cherry seedlings had leaf areas ranging from 25.4 to 60.3 cm2. Isozyme polymorphism was observed in seedling populations of both species at 7 enzyme loci with 35 alleles in total (19 alleles in cherries and 16 alleles in Mahaleb). Isozyme variability was higher in sweet cherries than Mahaleb due to high level of heterozygosity.
The study demonstrates the value of clonal rootstocks for eliminating the genetic variability within seedling rootstocks.
For this purpose, wild cherries (P. avium) and Mahaleb (P. mahaleb L.) have been used extensively.
The use of clonal (vegetatively propagated) rootstocks for sweet cherries is low in Turkey due to the high cost of nursery plants.
Seeds for rootstock propagation usually are collected by nurserymen from trees of unknown origin.
The objectives of this study were to compare the seedlings of two Prunus species for morphological characteristics and to detect any isozyme polymorphisms in seedling populations.
During the study, variations in germination rate, leaf area and seedling size were evaluated.
Isozyme variation was surveyed for five enzymes including alcohol dehydrogenase (ADH, EC 1.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.11.1.37), peroxidase (PRX, EC 1.11.1.7) and phosphoglucomutase (PGM, EC 2.7.5.1) in the populations.
Wild cherry (P. avium) seedlings exhibited greater variation than P. mahaleb. Cherry seedlings had leaf areas ranging from 25.4 to 60.3 cm2. Isozyme polymorphism was observed in seedling populations of both species at 7 enzyme loci with 35 alleles in total (19 alleles in cherries and 16 alleles in Mahaleb). Isozyme variability was higher in sweet cherries than Mahaleb due to high level of heterozygosity.
The study demonstrates the value of clonal rootstocks for eliminating the genetic variability within seedling rootstocks.
Publication
Authors
M. Şeker
Keywords
Prunus avium, P. mahaleb, propagation, rootstock, genotypic variation
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