Articles
IDENTIFICATION AND TAXONOMIC STATUS OF CHINESE DIOSPYROS SPP. (EBENACEAE) ANDROECIOUS GERMPLASMS
Article number
833_13
Pages
91 – 96
Language
English
Abstract
Some Diospyros spp. (Ebenaceae) androecious germplasms (bearing only staminate flowers) were recently discovered in Dabieshan region in central China.
The origin and the genetic backgrounds of those materials need to be further exploited.
On 29 accessions of Diospyros spp., including 8 androecious individuals, 8 Chinese and 11 Japanese genotypes of D. kaki Thunb.
RAPD analysis was performed.
There were 434 polymorphic bands of total 499 amplified fragments in the analysis.
Phylogenetic trees were generated using UPGMA clustering, minimum evolution (ME), wagner parsimony (WP) by similarity coefficient.
While similarities among different groups were less than 0.71 using UPGMA, 29 genotypes could be divided into 3 groups including Chinese genotypes, Japanese genotypes and outgroup.
The other two different clustering methods (ME and WP) showed similar results as those from UPGMA analysis.
The results showed that Chinese androecious genotypes had a close genetic relationship to Luotian-tianshi persimmon, meaning those accessions may share a common progenitor with Luotian-tianshi. Combined morphology, pollen characteristic and chromosome numbers studies supported that those D. kaki Thunb. accessions are androecious genotypes occurring in China.
The genetic relationship between Baogai-tianshi and Mopanshi persimmon was close. Xiangxi-tianshi persimmon had a high similarity coefficient with Japanese cultivars Maekawa-jirou. Differences among groups are much less than differences among accessions within groups, indicating that the diversity within groups was greater than between groups.
The origin and the genetic backgrounds of those materials need to be further exploited.
On 29 accessions of Diospyros spp., including 8 androecious individuals, 8 Chinese and 11 Japanese genotypes of D. kaki Thunb.
RAPD analysis was performed.
There were 434 polymorphic bands of total 499 amplified fragments in the analysis.
Phylogenetic trees were generated using UPGMA clustering, minimum evolution (ME), wagner parsimony (WP) by similarity coefficient.
While similarities among different groups were less than 0.71 using UPGMA, 29 genotypes could be divided into 3 groups including Chinese genotypes, Japanese genotypes and outgroup.
The other two different clustering methods (ME and WP) showed similar results as those from UPGMA analysis.
The results showed that Chinese androecious genotypes had a close genetic relationship to Luotian-tianshi persimmon, meaning those accessions may share a common progenitor with Luotian-tianshi. Combined morphology, pollen characteristic and chromosome numbers studies supported that those D. kaki Thunb. accessions are androecious genotypes occurring in China.
The genetic relationship between Baogai-tianshi and Mopanshi persimmon was close. Xiangxi-tianshi persimmon had a high similarity coefficient with Japanese cultivars Maekawa-jirou. Differences among groups are much less than differences among accessions within groups, indicating that the diversity within groups was greater than between groups.
Publication
Authors
Q. Zhang, D. Guo, Z. Luo
Keywords
persimmon, random amplified polymorphism (RAPD), phylogenetics
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