DIFFERENCES IN THREE-DIMENSIONAL DISTRIBUTION OF TANNIN CELLS IN FLESH TISSUE BETWEEN ASTRINGENT AND NON-ASTRINGENT TYPE PERSIMMON

The distribution patterns of tannin cells in flesh tissue of persimmon of a non-astringent type, ‘Jiro’, and an astringent type, ‘Yokono’ was investigated.
Samples were taken from equatorial portions of the mesocarp in both cultivars at one-month interval during fruit development from 26 May to 30 October.
Those were embedded in paraffin wax after fixation and the embedded blocks were serially sectioned at 8 to 23 µm thickness depending on developmental stages of the fruit.
Then, these sections were observed with light microscopy and approximately 90 images of each serial section were reconstructed as the three-dimensional image using a personal computer with DeltaViewer 2.1.1. When the three-dimensional images were analyzed at cell division stages, tannin cells were distributed densely and connected to each other as a continuous mass of tannin cells in both cultivars.
However, at cell enlargement stages, the continuity of tannin cells was disrupted and tannin cells distributed scatteringly in ‘Jiro’. The continuity of tannin cells in ‘Yokono’ remained at these stages and tannin cells still distributed densely.
The relationship between the continuity-loss of tannin cells and the stop of tannin cell enlargement in ‘Jiro’ was discussed.

IV International Symposium on Persimmon

K. Yonemori, Y. Suzuki

3-D reconstruction, tannin cell, distribution pattern, continuity

https://doi.org/10.17660/ActaHortic.2009.833.18