Articles
CLONING AND EXPRESSION ANALYSIS OF A NOVEL MALE FERTILITY-RELATED GENE IN PETUNIA HYBRID
Article number
977_37
Pages
313 – 319
Language
English
Abstract
A novel male fertility-related gene, Phmf2, was identified in SSH libraries constructed from abortive and normal flower buds of Petunia hybrid. Phmf2 was up-regulated in abortive flower buds.
The full-length cDNA of Phmf2 was obtained by PCR amplification using gene-specific primers.
It contains a 1,005-bp open reading frame, encoding 334 amino acids polypeptide.
Predicted molecular weight is 37241.7 Da and estimated isoelectric point is 6.37. Sequence analysis showed that Phmf2 shared the highest similarity (91%) in amino acid sequence with previously recorded TKPR1 gene in Nicotiana tabacum and had the same high homology (72%) with DRL1 gene in Medicago (MtDFR) and Arabidopsis (AtDRL1), all of which are essential for male fertility.
Expression analysis by semi-quantitative RT-PCR showed that this gene highly expressed in abortive flower buds with none or little detected in normal flower buds or other organs.
We thus predicted that Phmf2 should be male fertility-related and hope it could be used to create a male sterile line of Petunia hybrid or other ornamentals, so as to play a significant role in ornamentals breeding.
The full-length cDNA of Phmf2 was obtained by PCR amplification using gene-specific primers.
It contains a 1,005-bp open reading frame, encoding 334 amino acids polypeptide.
Predicted molecular weight is 37241.7 Da and estimated isoelectric point is 6.37. Sequence analysis showed that Phmf2 shared the highest similarity (91%) in amino acid sequence with previously recorded TKPR1 gene in Nicotiana tabacum and had the same high homology (72%) with DRL1 gene in Medicago (MtDFR) and Arabidopsis (AtDRL1), all of which are essential for male fertility.
Expression analysis by semi-quantitative RT-PCR showed that this gene highly expressed in abortive flower buds with none or little detected in normal flower buds or other organs.
We thus predicted that Phmf2 should be male fertility-related and hope it could be used to create a male sterile line of Petunia hybrid or other ornamentals, so as to play a significant role in ornamentals breeding.
Authors
Y.Z. Yue, F.F. Ma, X. Huang, G.F. Liu, M.Z. Bao, H.R. Hu
Keywords
Phmf2, suppression subtractive hybridization, open reading frame, TKPR1, DRL1, semi-quantitative RT-PCR
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