TISSUE CULTURE AND REGENERATION OF PAEONIA SUFFRUTICOSA ANDR.

This paper was to study tissue culture technology of a Chinese tree peony ‘Luo Yang Hong’, and established in vitro regeneration system initially.
With the combination of different kinds of treatments, the effects of basic medium, plant growth regulators, and some other important factors on shoots multiplication, root induction, callus induction and differentiation were studied.
The main results showed as follows: the optimum multiplication medium of peony ‘Luo Yang Hong’ was PM + 0.5-1.5 mg•L^-1 6-BA + 25 g•L^-1 sucrose, rooting medium was 1/2PM + 15.0 mg•L^-1 IBA + 30 g•L^-1 sucrose, callus induction medium from the petiole as the explant was PM + 2.0-3.0 mg•L^-1 6-BA + 4.0 mg•L^-1 2,4-D + 0.1-0.5 mg•L^-1 NAA + 30 g•L^-1 sucrose, callus differentiation medium was PM + 2.0 mg•L^-1 6-BA + 1.0 mg•L^-1 2,4-D + 500 mg•L^-1 LH or CH + 0.01 mg•L^-1 TDZ + 45 g•L^-1 sucrose.

International Conference on Germplasm of Ornamentals

J.Q. Zhang, X.N. Jiang, X.F. Luo

Paeonia suffruticosa Andr., tissue culture, regeneration

https://doi.org/10.17660/ActaHortic.2013.977.50