Articles
Potential of common chemical fertilizers for micropropagation of Rhynchostylis gigantea (Lindl) Ridl.
Article number
1113_10
Pages
73 – 78
Language
English
Abstract
Rhynchostylis gigantea (Lindl) Ridl. is a native orchid in Thailand, Myanmar, Malaysia, Laos, Cambodia, Vietnam, China, Borneo, Bangladesh and The Philippines.
It is also commonly called foxtail orchid because of its long, thin, densely-packed inflorescence with sweetly fragrant blooms.
There are only a few Rhynchostylis remaining in nature and propagation through tissue culture techniques is required to increase the quantity.
Due to the high cost of the chemicals needed for tissue culture, the objective of this study was to reduce the production cost by investigating the effects of 3 types of common orchid fertilizers, i.e., 16-21-27 (N-P2O5-K2O), 21-21-21 (N-P2O5-K2O) and the commercial product LSQUOGoemarRSQUO, compared with the standard medium of Vacin and Went (VW). We cultured 0.2 g of protocorm-like bodies (Plbs) on 10 media in both solid and liquid conditions for 5 weeks.
The results showed that the medium that contained 1,500 ppm of 16-21-27 was the best for plantlet induction and fresh weight of Plbs in liquid conditions (4.42 plants vessel-1; 2.30 Plbs vessel-1). On the solid conditions, 1,500 ppm of 21-21-21 was the best condition to induce plantlets (3.90 plants vessel-1). In general, plantlet induction under liquid conditions was better than solid conditions with all the tested media.
The media containing the 16-21-27 chemical fertilizer at the concentration of 1,500 ppm in liquid conditions, and the 21-21-21 at a concentration of 1,500 ppm in solid conditions have the potential to replace the standard VW orchid medium.
It is also commonly called foxtail orchid because of its long, thin, densely-packed inflorescence with sweetly fragrant blooms.
There are only a few Rhynchostylis remaining in nature and propagation through tissue culture techniques is required to increase the quantity.
Due to the high cost of the chemicals needed for tissue culture, the objective of this study was to reduce the production cost by investigating the effects of 3 types of common orchid fertilizers, i.e., 16-21-27 (N-P2O5-K2O), 21-21-21 (N-P2O5-K2O) and the commercial product LSQUOGoemarRSQUO, compared with the standard medium of Vacin and Went (VW). We cultured 0.2 g of protocorm-like bodies (Plbs) on 10 media in both solid and liquid conditions for 5 weeks.
The results showed that the medium that contained 1,500 ppm of 16-21-27 was the best for plantlet induction and fresh weight of Plbs in liquid conditions (4.42 plants vessel-1; 2.30 Plbs vessel-1). On the solid conditions, 1,500 ppm of 21-21-21 was the best condition to induce plantlets (3.90 plants vessel-1). In general, plantlet induction under liquid conditions was better than solid conditions with all the tested media.
The media containing the 16-21-27 chemical fertilizer at the concentration of 1,500 ppm in liquid conditions, and the 21-21-21 at a concentration of 1,500 ppm in solid conditions have the potential to replace the standard VW orchid medium.
Authors
S. Neera, D. Boungphengphanh
Keywords
Rhynchostylis gigantea (Lindl) Ridl., orchid, chemical fertilizer, protocorm-like bodies, plantlets
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