Investigation on micropropagation of Staphylea pinnata L., a rare protected ornamental plant

The objective of the present study was to develop a micropropagation protocol for Staphylea pinnata. Shoots of the bladdernut were collected in February, from plants growing in The Plant Collection near Krakow, in Poland.
The buds were cut from the middle section of the shoots and were surface disinfected with 70% ethanol (30 s), followed by application of 0.75% sodium hypochlorite for 15 min, washing 3 times in sterile water, and finally in 0.08% sodium hypochlorite for 2-3 s.
The shoot tips were isolated from the buds and were cultured on the following media: Murashise and Skoog (MS), Quoirin and Lepoivre or Woody Plant Medium, all modified by addition of FeEDDHA (20 mg L^-1). Different combinations of plant growth regulators were tested: 6-benzylaminopurine (BAP) at 5.0-8.0 µM, gibberellic acid at 1.5 µM, 1-naphthaleneacetic acid (NAA) at 0.5 µM, and thidiazuron (TDZ) at 1.0 µM. The disinfection efficiency was 89.2%, and more than 30% of shoot tips cultured on the MS medium with 5.0 µM BAP and 0.5 µM NAA developed axillary buds.
The highest multiplication rate was observed on the MS medium supplemented with FeEDDHA and 5.0 µM BAP, 0.5 µM NAA and 1.0 µM TDZ.

XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques

B. Szewczyk-Taranek, B. Pawlowska

in vitro, shoot multiplication, BAP, thidiazuron, FeEDDHA

https://doi.org/10.17660/ActaHortic.2016.1113.30