Articles
A management system for tracking high health in vitro kiwifruit germplasm
Article number
1113_16
Pages
113 – 118
Language
English
Abstract
The maintenance of in vitro germplasm is an integral part of Plant & Food ResearchRSQUOs extensive kiwifruit breeding programme.
To manage the large numbers of plants and to fulfil biosecurity requirements, an auditable tissue tracking system is essential for capturing relevant data on the initiation, growth, multiplication, storage and movements of tissue, in addition to details on pathogen screening for Pseudomonas syringae pv. actinidiae (Psa). A customised database program (Visual Studio®) links to an independent database providing significant efficiency gains over ad hoc spreadsheet-based systems.
The system tracks activities applied to tissue, issuing unique ‘Activity Event ID’ codes which allow a history of events to be generated.
Data input from culture vessels starts by scanning the unique Activity Event ID on the mother vessels and adding relevant information.
Manual data input is minimised.
As culture vessels are processed, new labels, complete with barcodes, are generated for new culture vessels.
Labels contain readable information and are flagged to indicate if tissue can be certified as clean following pathogen screening.
Reports, which include the history of any culture vessel, are instantly available from the scanned bar codes.
The design is sufficiently flexible to use it with other germplasm collections especially where biosecurity requirements require auditable tracking of plant material.
To manage the large numbers of plants and to fulfil biosecurity requirements, an auditable tissue tracking system is essential for capturing relevant data on the initiation, growth, multiplication, storage and movements of tissue, in addition to details on pathogen screening for Pseudomonas syringae pv. actinidiae (Psa). A customised database program (Visual Studio®) links to an independent database providing significant efficiency gains over ad hoc spreadsheet-based systems.
The system tracks activities applied to tissue, issuing unique ‘Activity Event ID’ codes which allow a history of events to be generated.
Data input from culture vessels starts by scanning the unique Activity Event ID on the mother vessels and adding relevant information.
Manual data input is minimised.
As culture vessels are processed, new labels, complete with barcodes, are generated for new culture vessels.
Labels contain readable information and are flagged to indicate if tissue can be certified as clean following pathogen screening.
Reports, which include the history of any culture vessel, are instantly available from the scanned bar codes.
The design is sufficiently flexible to use it with other germplasm collections especially where biosecurity requirements require auditable tracking of plant material.
Authors
J.F. Seelye, S. Corpe, M.C. Debenham
Keywords
Actinidia, bar-coding, conservation, data management, Pseudomonas syringae, software
Online Articles (40)