Articles
Nutrition in fed-batch bioreactors affects subsequent size and productivity of turmeric during six months in greenhouse
Article number
1113_8
Pages
59 – 66
Language
English
Abstract
The effect of in vitro plant density and mineral concentrations on subsequent greenhouse growth was examined using a response surface method.
In vitro plants of turmeric were propagated in 35 different treatments of nutrient elements P (1.25-6.25 mM), Ca (3-9 mM), Mg (1.5-4.5 mM), KNO3 (20-60 mM) and plant density factors (6-18 buds per vessel), selected by D-optimal criteria and cultured over 5 months in intermittent-immersion rocker bioreactors, using mineral nutrients plus sucrose fed-batch technique.
Plants were transferred to greenhouse and grown for 6 months.
Plants grown in high density (18 buds vessel-1) in vitro culture produced more new plants, had the highest relative fresh growth and the largest rhizome dry mass compared to low density (6 buds vessel-1) with similar concentrations of mineral in vitro.
The plants grown in vitro with moderate KNO3 (37.7 mM) produced 8±0.9 new plants stock plant-1 in greenhouse.
Moderate P (3.5-4 mM) and Ca (5.7-6 mM), lowest Mg (1.5 mM), and highest KNO3 (60 mM) showed greatest mass increase (26.6±2.3 fold stock plant-1) and greatest rhizome production (50.7±2.8 g dry mass stock plant-1) in greenhouse.
Secondary rhizomes were observed in all treatments.
A multifactor design in vitro resolved combined effects of plant density and several elements during subsequent growth in one greenhouse experiment.
High in vitro plant density combined with proper mineral nutrition yielded more plants that continue to grow better during a season of greenhouse growth.
Optimization for propagation stock has a different nutrient mixture than optimization for biomass and rhizomes.
In vitro plants of turmeric were propagated in 35 different treatments of nutrient elements P (1.25-6.25 mM), Ca (3-9 mM), Mg (1.5-4.5 mM), KNO3 (20-60 mM) and plant density factors (6-18 buds per vessel), selected by D-optimal criteria and cultured over 5 months in intermittent-immersion rocker bioreactors, using mineral nutrients plus sucrose fed-batch technique.
Plants were transferred to greenhouse and grown for 6 months.
Plants grown in high density (18 buds vessel-1) in vitro culture produced more new plants, had the highest relative fresh growth and the largest rhizome dry mass compared to low density (6 buds vessel-1) with similar concentrations of mineral in vitro.
The plants grown in vitro with moderate KNO3 (37.7 mM) produced 8±0.9 new plants stock plant-1 in greenhouse.
Moderate P (3.5-4 mM) and Ca (5.7-6 mM), lowest Mg (1.5 mM), and highest KNO3 (60 mM) showed greatest mass increase (26.6±2.3 fold stock plant-1) and greatest rhizome production (50.7±2.8 g dry mass stock plant-1) in greenhouse.
Secondary rhizomes were observed in all treatments.
A multifactor design in vitro resolved combined effects of plant density and several elements during subsequent growth in one greenhouse experiment.
High in vitro plant density combined with proper mineral nutrition yielded more plants that continue to grow better during a season of greenhouse growth.
Optimization for propagation stock has a different nutrient mixture than optimization for biomass and rhizomes.
Authors
R.F. El-Hawaz, J.W. Adelberg, W.C. Bridges
Keywords
Curcuma longa L., plant density, response surface design, media, nursery
Online Articles (40)