Articles
Elimination of Garlic common latent virus by meristem culture and chemotherapy
Article number
1113_35
Pages
233 – 238
Language
English
Abstract
Meristem isolation and chemotherapy were used for Garlic common latent virus (GCLV) elimination in garlic (Allium sativum L.). Five of GCLV infected garlic genotypes were chosen for the experiment.
Ribavirin at concentrations of 25 or 50 mg L-1 was used for chemotherapy.
The effects of chemotherapy and meristem isolation were assessed.
The presence of the virus was indexed by ELISA tests.
The best result for GCLV elimination was chemotherapy using 25 mg L-1 of ribavirin.
In this treatment, GCLV was eliminated in 100% of the plants of ‘N9A’, ‘Anton’, and ‘Tristan’ genotypes.
In ‘Tristan’ and ‘Anton’ 100% of plantlets were indexed virus negative after using 50 mg L-1 of ribavirin.
No virus free plant was achieved in the case of genotype ‘Mako’ using meristem isolation.
The highest number of plants negatively indexed for GCLV was achieved using 25 mg L-1 ribavirin.
Ribavirin at concentrations of 25 or 50 mg L-1 was used for chemotherapy.
The effects of chemotherapy and meristem isolation were assessed.
The presence of the virus was indexed by ELISA tests.
The best result for GCLV elimination was chemotherapy using 25 mg L-1 of ribavirin.
In this treatment, GCLV was eliminated in 100% of the plants of ‘N9A’, ‘Anton’, and ‘Tristan’ genotypes.
In ‘Tristan’ and ‘Anton’ 100% of plantlets were indexed virus negative after using 50 mg L-1 of ribavirin.
No virus free plant was achieved in the case of genotype ‘Mako’ using meristem isolation.
The highest number of plants negatively indexed for GCLV was achieved using 25 mg L-1 ribavirin.
Authors
M. Kudělková, E. Ondru¿iková, H. Sasková
Keywords
ribavirin, Allium sativum, GCLV, virus free plants, ELISA
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