Articles
Micropropagation of Teucrium capitatum L.
Article number
1113_27
Pages
183 – 188
Language
English
Abstract
The effect of explant origin on micropropagation of Teucrium capitatum was studied.
Explants collected from in vitro grown seedlings and cultured initially on MS medium with 0.5-2.0 mg L-1 benzyladenine (BA) produced shoots at high percentage (80-87%). More shoots per explant (12.6) were formed on the medium with 2.0 mg L-1 BA and longer shoots (1.7 cm) on that with 0.5 mg L-1 BA. Followed subcultures on MS medium with 1.0 mg L-1 BA produced, on average, 7.6 shoots per explant.
Explants collected from adult native plants and cultured on MS medium with 1.0 mg L-1 BA were contaminated at high percentage and did not give any response.
Explants collected from plantlets grown from cuttings in a greenhouse were contaminated at low percentage (10%) when cultured on hormone-free MS medium or on MS medium supplemented with 1 mg L-1 BA or zeatin, but their response for shooting was low (13-25%), irrespectively of the applied treatment.
In the BA-supplemented medium more shoots per explant were produced (6.6), while zeatin induced the longest shoots (4.4 cm). Microshoots, that were cultured for root induction for 5 weeks on half-strength MS medium with various IBA concentrations (0.0-2.0 mg L-1), rooted at higher percentages (31-40%) on media containing indole-3-butyric acid (ΙΒΑ), compared to the IBA-free medium (11%). Plantlets were acclimatized to ex vitro conditions at 82% on a peat:perlite (1:1, v/v) substrate.
Explants collected from in vitro grown seedlings and cultured initially on MS medium with 0.5-2.0 mg L-1 benzyladenine (BA) produced shoots at high percentage (80-87%). More shoots per explant (12.6) were formed on the medium with 2.0 mg L-1 BA and longer shoots (1.7 cm) on that with 0.5 mg L-1 BA. Followed subcultures on MS medium with 1.0 mg L-1 BA produced, on average, 7.6 shoots per explant.
Explants collected from adult native plants and cultured on MS medium with 1.0 mg L-1 BA were contaminated at high percentage and did not give any response.
Explants collected from plantlets grown from cuttings in a greenhouse were contaminated at low percentage (10%) when cultured on hormone-free MS medium or on MS medium supplemented with 1 mg L-1 BA or zeatin, but their response for shooting was low (13-25%), irrespectively of the applied treatment.
In the BA-supplemented medium more shoots per explant were produced (6.6), while zeatin induced the longest shoots (4.4 cm). Microshoots, that were cultured for root induction for 5 weeks on half-strength MS medium with various IBA concentrations (0.0-2.0 mg L-1), rooted at higher percentages (31-40%) on media containing indole-3-butyric acid (ΙΒΑ), compared to the IBA-free medium (11%). Plantlets were acclimatized to ex vitro conditions at 82% on a peat:perlite (1:1, v/v) substrate.
Authors
A.N. Martini, M. Papafotiou, D.A.N. Majumder
Keywords
acclimatization, cytokinins, medicinal plant, Mediterranean plant, native ornamental, rooting, shoot multiplication
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