Micropropagation of Limoniastrum monopetalum L.

Shoot-tip explants collected from adult Limoniastrum monopetalum plants in spring or summer were established in vitro at high percentages (81 and 100%, respectively). At the establishment stage more shoots per explant (7.1) were produced on MS medium supplemented with 1.0 mg L^-1 benzyladenine (ΒΑ) or with 1.0 mg L^-1 zeatin (ZEA) (2.2 shoot explant^-1), compared to hormone-free medium (1.0 shoot explant^-1), whereas shoots were longer in the hormone-free medium (3.3 cm). In the hormone-free and ZEA-containing media, roots were formed at high percentages (95 and 69%, respectively). During subculture on MS with various ΒΑ concentrations (0.5-4.0 mg L^-1), shoots were formed at relatively higher percentage in the medium with 0.5 mg L^-1 ΒΑ (100%) and at lower percentage with 4.0 mg L^-1 ΒΑ (80%). Explants cultured on hormone-free medium did not form axillary shoots, just elongated and formed roots at the percentage of 87%. In the medium with 0.5 mg L^-1 ΒΑ, the most (8.5) and the longest shoots (0.8 cm) were formed, whereas as the BA concentration increased the number and length of shoots produced diminished.
Microshoots cultured on half-strength MS medium with 0.5-4.0 mg L^-1 indole-3-acetic acid (ΙΒΑ) rooted at the higher percentages (88-100%) and formed more roots per microshoot (3.4-7.6), compared to the hormone-free medium (43% and 1.6, respectively), but root length was higher in the hormone-free medium (2.1 cm) compared to ΙΒΑ (0.5-0.9 cm). Nearly 90% of plantlets were successfully acclimatized to ex vitro conditions on a mixture of peat-perlite (1:1, v/v).

XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques

A.N. Martini, M. Papafotiou

halophyte, medicinal plant, acclimatization, cytokinins, native ornamental, rooting, shoot multiplication, Mediterranean plant

https://doi.org/10.17660/ActaHortic.2016.1113.29