Articles
Effects of medium constituents and vessel ventilation on the in vitro flowering of Gypsophila muralis L.
Article number
1113_24
Pages
165 – 170
Language
English
Abstract
In this study we investigated the effects of medium constituents and vessel ventilation on the in vitro flowering of an annual gypsophila.
Seeds of Gypsophila muralis L. ‘Gypsy Deep Rose’ were sown on ¼-strength MS medium supplemented with 30 g L-1 sucrose and 8 g L-1 agar (pH 5.8). Seedlings were raised under 16 h light (40 μmol m-2 s-1 PPF with cool white fluorescent lamps)/8 h dark at 23°C. Seedlings at the 4 true-leaf unfolding stage were selected and subcultured into the various modified MS and Kano media and to ½ MS medium with sucrose concentrations from 0-80 g L-1. They were cultured under the above-mentioned conditions.
The best in vitro growth and flowering were obtained when seedlings were cultured on the ½ MS supplemented with 40 g L-1 sucrose (flower inducing medium). Using the flower inducing medium, we examined the effect on flowering of different durations of increased ventilation via a membrane filter (MilliSeal) commencing between 0-6 weeks from seedling subculture.
Increasing the rate of vessel ventilation caused incremental increases in shoot growth and floret numbers.
The best result was obtained when ventilation started 2-4 weeks after subculture; 100% in vitro flowering of the annual gypsophila was induced within 50-60 days after sowing.
Also, the decorative period of the flowers was at least 50 days under a general indoor environment.
Moreover, the exclusion of ammonium-form nitrogen from the media inhibited flowering in the gypsophila.
These results are unique compared to other plants reported in the past.
Seeds of Gypsophila muralis L. ‘Gypsy Deep Rose’ were sown on ¼-strength MS medium supplemented with 30 g L-1 sucrose and 8 g L-1 agar (pH 5.8). Seedlings were raised under 16 h light (40 μmol m-2 s-1 PPF with cool white fluorescent lamps)/8 h dark at 23°C. Seedlings at the 4 true-leaf unfolding stage were selected and subcultured into the various modified MS and Kano media and to ½ MS medium with sucrose concentrations from 0-80 g L-1. They were cultured under the above-mentioned conditions.
The best in vitro growth and flowering were obtained when seedlings were cultured on the ½ MS supplemented with 40 g L-1 sucrose (flower inducing medium). Using the flower inducing medium, we examined the effect on flowering of different durations of increased ventilation via a membrane filter (MilliSeal) commencing between 0-6 weeks from seedling subculture.
Increasing the rate of vessel ventilation caused incremental increases in shoot growth and floret numbers.
The best result was obtained when ventilation started 2-4 weeks after subculture; 100% in vitro flowering of the annual gypsophila was induced within 50-60 days after sowing.
Also, the decorative period of the flowers was at least 50 days under a general indoor environment.
Moreover, the exclusion of ammonium-form nitrogen from the media inhibited flowering in the gypsophila.
These results are unique compared to other plants reported in the past.
Authors
M. Shimizu, W. Amaki
Keywords
ammonium-form nitrogen, low baby’s breath, medium strength, number of air exchanges per hour, sucrose concentration
Online Articles (40)