Propagation of Phalaenopsis ‘Puspa Tiara Kencana’ via somatic embryogenesis

In vitro propagation study of Phalaenopsis LSQUOPuspa Tiara KencanaRSQUO via somatic embryogenesis (SE) was successfully performed.
Young leaf explants as the suitable explant produced embryogenic callus (EC) up to 19.9%, and 8.8% of them produced somatic embryos (SEs) directly on half-strength MS medium, supplemented with 0.5 mg L^-1 of N⁶-benzylaminopurine (BAP) and 1 mg L^-1 of thidiazuron (TDZ) within 38 days after initiation.
EC readily developed on the leaf explants 3.25 g EC weight and 0.63 cm³ EC volume, on average, while half-strength MS medium supplemented with 0.5 mg L^-1 BAP and 1 mg L^-1 TDZ stimulated 0.7 g EC weight and 3.1 cm³ EC volume.
EC grew and proliferated optimally in leaf explant with a callus growth of 2.2 g month^-1, and on the New Phalaenopsis (NP) medium, supplemented with 1 mg L^-1 TDZ and 0.5 mg L^-1 BAP, with EC growth of 1.6 g month^-1. After 12 weeks of culture, 20 germinated SEs per clump were successfully established on NP medium with 0.05 mg L^-1 of BAP, while more vigorous and greener plantlets were determined on half-strength MS medium, containing 0.05 mg L^-1 BAP.

XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques

D. Pramanik, F. Rachmawati, B. Winarto

moth orchid, explants, media, in vitro culture, somatic embryos

https://doi.org/10.17660/ActaHortic.2016.1113.3