Articles
Plant regeneration from nodal explants of ‘Irwin’ mango seedlings
Article number
1113_18
Pages
127 – 134
Language
English
Abstract
Micropropagation of seedlings of ‘Irwin’, a Japanese leading and monoembryonic mango (Mangifera indica L.) cultivar, was studied.
During culture establishment, prevention of contamination of explants from ex vitro shoots is one of key success factors of mango micropropagation.
Hence, we used seedlings raised in the laboratory for explant which greatly reduced contamination and increased explant survival and establishment.
Among the different basal media used, full- or half-strength woody plant media (WP or ½WP) produced better shoot growth than full- or half-strength MS media (MS or ½MS). Zeatin promoted shoot elongation but not shoot multiplication, whereas 6-benzyladenine (BA) had the opposite effect.
Hence, a combination of zeatin and BA was used for shoot proliferation.
Indolebutyric acid (IBA) treatment promoted rooting of microcuttings, although it inhibited root-elongation.
About 90% of microcuttings treated with IBA for 3 days in the dark rooted.
Fine vermiculite was found to be the best potting medium for micropropagated mango plantlets, although the growth of rooted microcuttings was poor after potting.
During culture establishment, prevention of contamination of explants from ex vitro shoots is one of key success factors of mango micropropagation.
Hence, we used seedlings raised in the laboratory for explant which greatly reduced contamination and increased explant survival and establishment.
Among the different basal media used, full- or half-strength woody plant media (WP or ½WP) produced better shoot growth than full- or half-strength MS media (MS or ½MS). Zeatin promoted shoot elongation but not shoot multiplication, whereas 6-benzyladenine (BA) had the opposite effect.
Hence, a combination of zeatin and BA was used for shoot proliferation.
Indolebutyric acid (IBA) treatment promoted rooting of microcuttings, although it inhibited root-elongation.
About 90% of microcuttings treated with IBA for 3 days in the dark rooted.
Fine vermiculite was found to be the best potting medium for micropropagated mango plantlets, although the growth of rooted microcuttings was poor after potting.
Authors
T. Tetsumura, T. Sakota, H. Nagano, S. Izaka, T.M.H. Nguyen, S. Tamura, S. Ishimura, C. Honsho
Keywords
basal medium, contamination, cytokinin, Mangifera indica, micropropagation
Online Articles (40)