Articles
Effects of chemical fertilizer medium on tissue culture of strawberry
Article number
1285_11
Pages
69 – 74
Language
English
Abstract
Strawberry is fruit rich in nutrient, useful for health for the content in valuable compounds, such as vitamins A, C, B1, B2, protein, calcium, potassium, copper, iron, and especially antioxidants.
Strawberry is a very popular fruit because the antioxidants in its fruits are much more than in tomatoes, i.e., up to 7 times.
Presently, propagation of strawberry cultivars requires tissue culture.
Because of diseases, infection from the mother plant results in low productivity, and chemicals used in vitro are expensive.
The aim of this experiment was to replace the chemical fertilizer recipe, affecting the in vitro growth of strawberry.
The experiment was conducted using a 5×4 factorial design with the cultivar Royal 80. Explants were cultured in media containing 5 types of chemical fertilizer, 21-21-21 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O), 36-5-5 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O) and 10-52-17 (N-P2O5-K2O) at concentrations of 0.5, 1.5, 2.5 and 3.5 g L‑1, compared with MS medium used as the control in each type of fertilizer.
Culture in 0.5 g L‑1 of 21-21-21 showed the highest number of plantlets (4.3 plantlets explant‑1), higher than the culture in MS medium (3.44 plantlets explant‑1), 15-30-15 and 36-5-5 media (3.48 and 3.87 plantlets explant‑1, respectively)
Strawberry is a very popular fruit because the antioxidants in its fruits are much more than in tomatoes, i.e., up to 7 times.
Presently, propagation of strawberry cultivars requires tissue culture.
Because of diseases, infection from the mother plant results in low productivity, and chemicals used in vitro are expensive.
The aim of this experiment was to replace the chemical fertilizer recipe, affecting the in vitro growth of strawberry.
The experiment was conducted using a 5×4 factorial design with the cultivar Royal 80. Explants were cultured in media containing 5 types of chemical fertilizer, 21-21-21 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O), 36-5-5 (N-P2O5-K2O), 15-30-15 (N-P2O5-K2O) and 10-52-17 (N-P2O5-K2O) at concentrations of 0.5, 1.5, 2.5 and 3.5 g L‑1, compared with MS medium used as the control in each type of fertilizer.
Culture in 0.5 g L‑1 of 21-21-21 showed the highest number of plantlets (4.3 plantlets explant‑1), higher than the culture in MS medium (3.44 plantlets explant‑1), 15-30-15 and 36-5-5 media (3.48 and 3.87 plantlets explant‑1, respectively)
Authors
S. Neera, J. Pimlee
Keywords
strawberry, antioxidants, chemical fertilizers, plantlet, in vitro
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