Effect of culture media on in vitro culture of Rhynchostylis coelestis

Rhynchostylis coelestis Rchb.f. ex A.H. Kent is an orchid native to Thailand that currently needs to be conserved by in vitro culture technique since it is a vulnerable and endangered species.
The effect of different media and organic additives on in vitro propagation of Rhynchostylis coelestis Rchb.f. ex A.H. Kent were studied.
The best medium for induction of protocorm-like bodies (PLBs) and plantlet development were ½ VM combined from VW (Vacin and Went, 1949) and MS (Murashige and Skoog, 1962) and ½ MS supplemented with 50 g L^‑1 potato homogenate, 50 g L^‑1 banana pulp, 150 mL L^‑1 coconut water and 2 g L^‑1 activated charcoal.
These two culture media increased the weight of PLBs in 8 weeks by 28 and 30 times and increased the weight of plantlets by 7 and 6 times in 12 weeks.
The fresh weight of PLBs and plantlets was significantly different with additional 3 treatments; VW medium supplement with 150 mL L^‑1 coconut water, orchid fertilizer 21-21-21 2 g L^‑1 and chemical fertilizer 16-16-16 2 g L^‑1 substituting mineral salts of culture media.

XXX International Horticultural Congress IHC2018: II International Symposium on Micropropagation and In Vitro Techniques

T. Pithayawutwinit, W. Chinachit

Rhynchostylis coelestis, in vitro culture, organic additive

https://doi.org/10.17660/ActaHortic.2020.1285.7