Articles
In vitro culture establishment and plant regeneration of Arbutus unedo L.
Article number
1285_17
Pages
109 – 112
Language
English
Abstract
Arbutus unedo L. (strawberry tree) grows in the coastal Mediterranean region naturally.
This fruit tree receives a growing interest in the world as a result of common uses in the industrial, pharmaceutical and chemical fields.
In the present study, the shoot-tip culture and nodal segments of pre-selected three strawberry tree types were used for in vitro culture.
Naturally growing adult-plants were pruned in the autumn.
Newly growing shoots (5-10 cm length) were collected to obtain the shoot tip and nodal segment explants for the experiments in the following spring and early summer months.
Among the investigated criteria, sterilization procedure, genotype of the donor plants, concentrations of plant growth regulators were evaluated.
In the establishment and propagation stages, two basal medium MS and WPM were used.
The role of 6-benzylaminopurine (BAP) alone or with indole-3-butyric acid (IBA) on shoot proliferation, and the effect of IBA on root formation are evaluated.
This fruit tree receives a growing interest in the world as a result of common uses in the industrial, pharmaceutical and chemical fields.
In the present study, the shoot-tip culture and nodal segments of pre-selected three strawberry tree types were used for in vitro culture.
Naturally growing adult-plants were pruned in the autumn.
Newly growing shoots (5-10 cm length) were collected to obtain the shoot tip and nodal segment explants for the experiments in the following spring and early summer months.
Among the investigated criteria, sterilization procedure, genotype of the donor plants, concentrations of plant growth regulators were evaluated.
In the establishment and propagation stages, two basal medium MS and WPM were used.
The role of 6-benzylaminopurine (BAP) alone or with indole-3-butyric acid (IBA) on shoot proliferation, and the effect of IBA on root formation are evaluated.
Authors
M. Sulusoglu Durul, S. Memiş
Keywords
in vitro propagation, regeneration, rooting, browning, strawberry tree
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