Articles
Encapsulation of in vitro-derived propagules of two genotypes of Capparis spinosa (L.) from Pantelleria Island
Article number
1285_6
Pages
39 – 44
Language
English
Abstract
Encapsulation technology, combining the advantages of zygotic or gamic seed with those of micropropagation, has recently attracted the interest of researchers as a new propagation approach, mainly due to the unsatisfying results of the traditional propagation strategies.
The encapsulation of uninodal microcuttings (3-4 mm long) from two Sicilian Capparis spinosa (L.) genotypes (from Pantelleria Island: Tracino and Scauri) was evaluated, observing the influence of the calcium alginate coating and of three different growth regulators (PGRs): 6-benzylaminopurine (BAP), meta-topolin (MT) and zeatin (ZEA), on viability, regrowth and conversion of the propagules.
Caper microcuttings were dissected and placed in different Murashige and Skoog-based artificial endosperms, enriched of either MT, ZEA or BAP. The synthetic seeds obtained were sown on a medium with full strength salts concentration, enriched with 0.4 mg L‑1 of naphtalene acetic acid and 0.7 mg L‑1 of gibbelleric acid.
After 60 days, the following parameters were detected: viability, regrowth, number and length of the shoots and roots, conversion.
The results confirm that encapsulation did not negatively affect the viability, which showed the highest percentage with BAP (100%) in Tracino and with ZEA (100%) in Scauri. Similar results were obtained in regrowth, with statistically significant differences among the three PGRs tested: Tracino showed the best regrowth on capsuled enriched with BAP (100%), Scauri with ZEA (100%). In addition, the synseed conversion was greatly affected by the PGR, and it was higher in artificial endosperm added with BAP in Tracino (56.6%) and ZEA in Scauri (23.3%) genotype.
The encapsulation of uninodal microcuttings (3-4 mm long) from two Sicilian Capparis spinosa (L.) genotypes (from Pantelleria Island: Tracino and Scauri) was evaluated, observing the influence of the calcium alginate coating and of three different growth regulators (PGRs): 6-benzylaminopurine (BAP), meta-topolin (MT) and zeatin (ZEA), on viability, regrowth and conversion of the propagules.
Caper microcuttings were dissected and placed in different Murashige and Skoog-based artificial endosperms, enriched of either MT, ZEA or BAP. The synthetic seeds obtained were sown on a medium with full strength salts concentration, enriched with 0.4 mg L‑1 of naphtalene acetic acid and 0.7 mg L‑1 of gibbelleric acid.
After 60 days, the following parameters were detected: viability, regrowth, number and length of the shoots and roots, conversion.
The results confirm that encapsulation did not negatively affect the viability, which showed the highest percentage with BAP (100%) in Tracino and with ZEA (100%) in Scauri. Similar results were obtained in regrowth, with statistically significant differences among the three PGRs tested: Tracino showed the best regrowth on capsuled enriched with BAP (100%), Scauri with ZEA (100%). In addition, the synseed conversion was greatly affected by the PGR, and it was higher in artificial endosperm added with BAP in Tracino (56.6%) and ZEA in Scauri (23.3%) genotype.
Authors
M.A. Germanà, F. Lesto, F. Lo Bosco, N. Iacuzzi, E.A. Ozudogru
Keywords
caper, synthetic seed, encapsulation technology, micropropagation
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