Articles
Obtaining polyploid cucumber (Cucumis sativus L.) plants by using different explants
Article number
1285_9
Pages
57 – 62
Language
English
Abstract
Polyploidy is an important method for plant breeding.
In this study, it has been attempted to develop an effective procedure for obtaining polyploid plants from different Cengelkoy type cucumber (Cucumis sativus L.) explants to use in modern breeding studies and plant biotechnology.
In our study, chromosomal doubling was performed by applying 0.1% colchicine in three different explant types such as seeds, lateral shoots and apical shoots.
Germination of the seeds was carried out in MS0 medium to obtain lateral and apical shoots.
After that these explants were transferred to MS + BAP (0.23 mg L‑1) + 0.1% colchicine and they were incubated for 7 days.
At the end of the 7 days, lateral and apical shoots were transfered to MS + BAP (0.23 mg L‑1) without colchicine.
On the other hand, the seeds explants were kept in the 0.1% colchicine solution for 24 h, then transferred to MS0 medium for germination.
After all explants rooting, ploidy level of the plants was determined by using stoma-chloroplast counting method.
In this study, mainly mixoploid plants (69 plants, 56.1%) were obtained.
Thirty-four mixoploid plants were obtained as a result of seeds colchicine application.
According to all explant usage methods, 29 tetraploid plants were formed in total and it was determined that this number was 23.6% when evaluated proportionally.
The most successful results were observed in lateral shoots (20 plant, 41.7%) in terms of tetraploid plants obtained.
Also, no tetraploid plant was obtained in seed method.
According to this study, lateral shoot application was found to be the most effective method for obtaining polyploid plants.
In this study, it has been attempted to develop an effective procedure for obtaining polyploid plants from different Cengelkoy type cucumber (Cucumis sativus L.) explants to use in modern breeding studies and plant biotechnology.
In our study, chromosomal doubling was performed by applying 0.1% colchicine in three different explant types such as seeds, lateral shoots and apical shoots.
Germination of the seeds was carried out in MS0 medium to obtain lateral and apical shoots.
After that these explants were transferred to MS + BAP (0.23 mg L‑1) + 0.1% colchicine and they were incubated for 7 days.
At the end of the 7 days, lateral and apical shoots were transfered to MS + BAP (0.23 mg L‑1) without colchicine.
On the other hand, the seeds explants were kept in the 0.1% colchicine solution for 24 h, then transferred to MS0 medium for germination.
After all explants rooting, ploidy level of the plants was determined by using stoma-chloroplast counting method.
In this study, mainly mixoploid plants (69 plants, 56.1%) were obtained.
Thirty-four mixoploid plants were obtained as a result of seeds colchicine application.
According to all explant usage methods, 29 tetraploid plants were formed in total and it was determined that this number was 23.6% when evaluated proportionally.
The most successful results were observed in lateral shoots (20 plant, 41.7%) in terms of tetraploid plants obtained.
Also, no tetraploid plant was obtained in seed method.
According to this study, lateral shoot application was found to be the most effective method for obtaining polyploid plants.
Authors
E. Demirel, F.B. Celikli
Keywords
Cucumis sativus L., cucumber, polyploid, tetraploid
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