Effect of induction media on callus formation in un-pollinated ovule culture of three melon cultivars

Production of inbred lines, such as double haploid plants which are produced from pollen or ovule culture, is an important tool in a crop breeding program.
Some factors affecting in vitro gynogenesis, including genotypes of donor plants and induction media on callus formation in the un-pollinated ovule culture of melon, were investigated.
A 3×3 factorial experiment in completely randomized design with 5 replications, 2 factors including 3 melon cultivars in Thailand (‘Green Net’, ‘Honeydew’ and ‘Pot Orange’) which were used as donor plants, and 3 induction media [M1, MS + 0.20 mg L^‑1 6-benzylaminopurine (BAP) and 0.40 mg L^‑1 1-naphthalene acetic acid (NAA); M2, MS + 0.30 mg L^‑1 BAP + 0.30 mg L^‑1 NAA; and M3, MS + 0.40 mg L^‑1 BAP + 0.60 mg L^‑1 NAA] was conducted.
Un-pollinated ovules from melon flowers at one day before flowering were used and transferred to induction media in dark condition for 4 days, then their explants were cultured on daily 16 h LED light and 8 h dark, 80 μmol m^‑2 s^‑1. Callus formation was measured after 6 weeks.
The interactions between the 2 factors were non-significant.
The callus formation potential of all the 3 melon cultivars showed to be significant.
The addition of 0.20 mg L^‑1 BAP and 0.40 mg L^‑1 NAA into the MS medium induced the highest percentage of explants with callus formation with an average of 75.54%. Callus formation percentages, achieved in melon cultivars, can be indicator of efficient production of doubled haploids of melon in the future.

XXX International Horticultural Congress IHC2018: II International Symposium on Micropropagation and In Vitro Techniques

N. Nitwatthanakul, A. Tiraumphon

6-benzylaminopurine, 1-naphthalene acetic acid, un-pollinated, ovule culture, melon

https://doi.org/10.17660/ActaHortic.2020.1285.16