Articles
In vitro direct and indirect regeneration of promising lavandin cultivars
Article number
1285_32
Pages
213 – 220
Language
English
Abstract
Lavandin (Lavandula × intermedia Emeric ex Loiseleur, Lamiaceae) is an economically valuable plant, superior to commercially grown lavender cultivars in yield and essential oil output.
Traditional vegetative propagation of lavandin is a very slow and laborious process.
Biotechnological methods addressed to propagation and cleaning up are promising for the development of the essential oil industry in southern Russia.
Plant samples from cultivars Rabat, Temp and clone Snezhnyi Bars were collected in the open field collection of the Nikita Botanical Gardens.
After sterilization, meristems 0.5-0.7 mm size and shoot nodal segments 1.0-1.5 cm size were excised and placed on different culture media with plant growth regulators.
Initiation of meristem development was observed after 6-7 days on the modified MS and B5 media.
After 21-28 days on the media with 0.5-1.0 mg L‑1 BAP or 0.5-1.0 mg L‑1 kinetin, with 0.05-0.1 mg L‑1 NAA and 0.1-0.25 mg L‑1 GA3 direct microshoot regeneration was noted in cultivars Rabat, Temp and clone Snezhnyi Bars. Callus was formed at the base of microshoots and from the leaf cuts and petioles.
Histological analysis of callus demonstrated its ability to regenerate morphogenic structures, adventitious buds and microshoots on MS medium with 1.0 mg L‑1 BAP + 0.25 mg L‑1 NAA; 0.75 mg L‑1 kinetin + 2.5 mg L‑1 adenine sulfate + 0.25 mg L‑1 NAA and 0.5 mg L‑1 TDZ + 0.05 mg L‑1 NAA + 0.5 mg L‑1 GA3. Plant regeneration efficiency increased due to indirect regeneration and spontaneous rooting.
In this paper, the main ways of morphogenesis in three lavandin cultivars were revealed: direct regeneration via adventitious shoot formation; indirect regeneration via callus, adventitious buds formation and mass micropropagation.
Traditional vegetative propagation of lavandin is a very slow and laborious process.
Biotechnological methods addressed to propagation and cleaning up are promising for the development of the essential oil industry in southern Russia.
Plant samples from cultivars Rabat, Temp and clone Snezhnyi Bars were collected in the open field collection of the Nikita Botanical Gardens.
After sterilization, meristems 0.5-0.7 mm size and shoot nodal segments 1.0-1.5 cm size were excised and placed on different culture media with plant growth regulators.
Initiation of meristem development was observed after 6-7 days on the modified MS and B5 media.
After 21-28 days on the media with 0.5-1.0 mg L‑1 BAP or 0.5-1.0 mg L‑1 kinetin, with 0.05-0.1 mg L‑1 NAA and 0.1-0.25 mg L‑1 GA3 direct microshoot regeneration was noted in cultivars Rabat, Temp and clone Snezhnyi Bars. Callus was formed at the base of microshoots and from the leaf cuts and petioles.
Histological analysis of callus demonstrated its ability to regenerate morphogenic structures, adventitious buds and microshoots on MS medium with 1.0 mg L‑1 BAP + 0.25 mg L‑1 NAA; 0.75 mg L‑1 kinetin + 2.5 mg L‑1 adenine sulfate + 0.25 mg L‑1 NAA and 0.5 mg L‑1 TDZ + 0.05 mg L‑1 NAA + 0.5 mg L‑1 GA3. Plant regeneration efficiency increased due to indirect regeneration and spontaneous rooting.
In this paper, the main ways of morphogenesis in three lavandin cultivars were revealed: direct regeneration via adventitious shoot formation; indirect regeneration via callus, adventitious buds formation and mass micropropagation.
Authors
I.V. Mitrofanova, N.P. Lesnikova-Sedoshenko, T.N. Kuzmina, S.V. Chelombit, O.V. Mitrofanova
Keywords
Lavandula × intermedia, meristem, adventitious microshoot, callus, organogenesis, histological analysis, regenerant, in vitro
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