Articles
The establishment of effective regeneration system and in vitro culture of Campanula
Article number
1285_2
Pages
9 – 14
Language
English
Abstract
Several species of Campanula genus have been cultivated as ornamental plants, and there is an increasing demand for more attractive and novel characteristics.
Optimal regeneration protocol is prerequisite for subsequent successful genetic manipulation and generation of new features.
In the current study, the optimal media composition and explant source for shoot regeneration of three Campanula cultivars, C. portenschlagiana Blue Ocean (BO) and Royal, C. carpatica Improved Blue Uniform (IBU) were determined.
The focus of this study was based on avoiding an over-supplementation of plant growth regulators (PGRs), especially the cytokinin thidiazuron (TDZ). The explants examined for shoot regeneration were petioles harvested from two different explant sources: 1) petioles of 3-month-old tissue-cultured plants, 2) petioles of etiolated nodal cuts.
To find out the best media composition for shoot regeneration, different naphthalene acetic acid (0.5, 1.0 and 2.0 mg L‑1) and TDZ (1.0, 2.0, 3.0 and 5.0 mg L‑1) concentrations were investigated.
According to the results, the most favourable explant source for harvesting petioles were etiolated nodal cuts of mature greenhouse plants.
The best shoot regeneration obtained from the harvested petioles of nodal cuts in the 3 cultivars were 85% in IBU, 65% in BO and 45.8% in Royal.
Optimal regeneration protocol is prerequisite for subsequent successful genetic manipulation and generation of new features.
In the current study, the optimal media composition and explant source for shoot regeneration of three Campanula cultivars, C. portenschlagiana Blue Ocean (BO) and Royal, C. carpatica Improved Blue Uniform (IBU) were determined.
The focus of this study was based on avoiding an over-supplementation of plant growth regulators (PGRs), especially the cytokinin thidiazuron (TDZ). The explants examined for shoot regeneration were petioles harvested from two different explant sources: 1) petioles of 3-month-old tissue-cultured plants, 2) petioles of etiolated nodal cuts.
To find out the best media composition for shoot regeneration, different naphthalene acetic acid (0.5, 1.0 and 2.0 mg L‑1) and TDZ (1.0, 2.0, 3.0 and 5.0 mg L‑1) concentrations were investigated.
According to the results, the most favourable explant source for harvesting petioles were etiolated nodal cuts of mature greenhouse plants.
The best shoot regeneration obtained from the harvested petioles of nodal cuts in the 3 cultivars were 85% in IBU, 65% in BO and 45.8% in Royal.
Authors
Z. Ghayoor Karimiani, C. Gehl, M. Serek
Keywords
explant source, PGRs, shoot regeneration, TDZ concentration, thidiazuron
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