Articles
SETIS™, a novel variant within the temporary immersion bioreactors
Article number
1285_37
Pages
253 – 258
Language
English
Abstract
The temporary immersion system SETIS consists of an upper recipient for in vitro plants and a connected lower recipient for liquid medium.
The combination of periodical short immersion with nutrients and regular aeration of the headspace allows fast in vitro growth of a large number of plant species.
Benefits and drawbacks of the system are discussed and points of attention and pitfalls during set-up and maintenance are shared.
To illustrate that temporary immersion system requires its own medium optimization, a culture of pear in SETIS was compared with a classical culture on semi-solid medium.
The combination of periodical short immersion with nutrients and regular aeration of the headspace allows fast in vitro growth of a large number of plant species.
Benefits and drawbacks of the system are discussed and points of attention and pitfalls during set-up and maintenance are shared.
To illustrate that temporary immersion system requires its own medium optimization, a culture of pear in SETIS was compared with a classical culture on semi-solid medium.
Authors
M. Lotfi, S.P.O. Werbrouck
Keywords
micropropagation, mass production, in vitro, TIS
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