Articles
Effect of desiccation on somatic embryo regeneration in date palm (Phoenix dactylifera L.) cultured in vitro
Article number
1285_20
Pages
125 – 130
Language
English
Abstract
This work studied somatic embryo formation, maturation and germination using partial desiccation treatment in either sealed test tubes or Plantform bioreactor vessels for different numbers of days.
After desiccation, embryogenic callus was transferred to a regeneration medium for 16 weeks.
During the study it was found that embryogenic callus subjected to desiccation in both test tubes and sterile bioreactor significantly increased somatic embryo formation, and germinated, when compared to sealed test tubes.
However, a perfuse numbers of somatic embryos with good synchronization were obtained whit the use of the TIS (temporary immersion system) system, containing 500 mL liquid MS medium, 0.1 mg L‑1 benzylaminopurine, 0.1 mg L‑1 2ip and 0.1 mg L‑1 kinetin, and a high level of sucrose (100 g L‑1).
After desiccation, embryogenic callus was transferred to a regeneration medium for 16 weeks.
During the study it was found that embryogenic callus subjected to desiccation in both test tubes and sterile bioreactor significantly increased somatic embryo formation, and germinated, when compared to sealed test tubes.
However, a perfuse numbers of somatic embryos with good synchronization were obtained whit the use of the TIS (temporary immersion system) system, containing 500 mL liquid MS medium, 0.1 mg L‑1 benzylaminopurine, 0.1 mg L‑1 2ip and 0.1 mg L‑1 kinetin, and a high level of sucrose (100 g L‑1).
Authors
A.H. Ali, M. Welander
Keywords
Plantform bioreactor, callus, somatic embryo, maturation, TIS system, Phoenix dactylifera
Online Articles (40)