Articles
Factors affecting in vitro bulblet growth of Lilium orientalis ‘Stargazer’ and ‘Casablanca’
Article number
1285_13
Pages
81 – 88
Language
English
Abstract
Lily is an excellent model to study the correlation between culture media and bulblet growth to provide a fundamental relationship for bulblet production in vitro.
Bulblet growth is the most important factor in understanding how lily bulblets regenerate and grow in vitro and the role of media on the growth mechanism in lilies.
The aim of this study was to examine the effect of different culture media on enhancing bulblet size and gain more information about bulblet production in vitro.
Hence, solid vs. semi-solid versus liquid static culture were investigated.
The results show that explants grown in liquid medium formed bulblets that had the best fresh weight, bulblet number, and root:shoot ratio compared with explants grown in semi-solid and solid media.
Furthermore, a linear relationship among the culture media irrespective of cultivar suggests culture medium significantly influences lily explant regeneration and bulblet production in vitro.
The results of bulblet performance including the root:shoot ratio indicate that bulblet growth in a liquid static culture was sustained by the renewal of liquid medium, thus avoiding nutrient depletion, and might be a useful technique for producing bulblets and enhancing their growth in vitro compared to bulblets grown on an agar solidified medium.
Bulblet growth is the most important factor in understanding how lily bulblets regenerate and grow in vitro and the role of media on the growth mechanism in lilies.
The aim of this study was to examine the effect of different culture media on enhancing bulblet size and gain more information about bulblet production in vitro.
Hence, solid vs. semi-solid versus liquid static culture were investigated.
The results show that explants grown in liquid medium formed bulblets that had the best fresh weight, bulblet number, and root:shoot ratio compared with explants grown in semi-solid and solid media.
Furthermore, a linear relationship among the culture media irrespective of cultivar suggests culture medium significantly influences lily explant regeneration and bulblet production in vitro.
The results of bulblet performance including the root:shoot ratio indicate that bulblet growth in a liquid static culture was sustained by the renewal of liquid medium, thus avoiding nutrient depletion, and might be a useful technique for producing bulblets and enhancing their growth in vitro compared to bulblets grown on an agar solidified medium.
Authors
S. Islam, Md. Zohurul Kadir Roni, K. Shimasaki
Keywords
Lilium sp., bulblet growth, correlation, culture media, in vitro
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