Establishment of a micropropagation method for in vitro rooting of Callistemon viminalis micro-cuttings

A micropropagation method was described for bottlebrush (Callistemon viminalis) using single node explants under in vitro conditions.
This method provided a rapid, reliable, repeatable mass propagation method.
Multiple shoots were induced from axillary buds on Murashige and Skoog (MS) and Anderson’s Rhododendron Medium (ARM) media containing different concentrations of 6-Benzylaminopurine (BAP) in combination with naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA). Both MS and ARM media were suitable for these goals and the ARM medium with BAP (2 mg L^‑1) and NAA (0.2 mg L^‑1) was the most effective medium for shoot multiplication, while the ARM medium is better than the MS medium for shoot proliferation.
In vitro regenerated shoots on half-strength, MS media supplemented with IBA (1.5 mg L^‑1) developed better roots (100%), while no significant difference was evident between half-strength MS and MS medium containing NAA (0.2 mg L^‑1). The rooted plantlets were successfully acclimatized and the most viability of rooted shoots (100%) was observed with MS media supplemented with IBA (1.5 mg L^‑1) and half-strength MS medium supplemented with NAA (0.2 mg L^‑1) also.

I International Conference and X National Horticultural Science Congress of Iran (IrHC2017)

S. Shokri, H. Zarei, M. Alizadeh

multiplication, tissue culture, single node, in vitro, bottlebrush

https://doi.org/10.17660/ActaHortic.2021.1315.39