Articles
Effect of plant growth regulators and explant type on in vitro shoot multiplication of Salvia officinalis
Article number
1327_50
Pages
373 – 380
Language
English
Abstract
Salvia officinalis, sage, is a strongly aromatic, perennial grayish shrub, up to 60 cm, usually with lilac flowers (May-July), which is found in garrigue, stony pastures, scrub, rocky places.
It is one of the most important species of the genus Salvia worldwide, as it is cultivated in many varieties as a medicinal and ornamental plant.
In the present study, the effect of plant growth regulators and explant type on in vitro shoot multiplication of this species was investigated, aiming to the development of a micropropagation protocol that could facilitate breeding and promotion of it for ornamental use.
Shoot-tip and nodal explants excised from in vitro grown seedlings were initially cultured on MS medium without plant growth regulators (control) or supplemented with 0.1 mg L‑1 6-benzyladenine (ΒΑ) and 0.0 or 0.01 mg L‑1 1-naphthaleneacetic acid (NAA). In the following subculture, an additional medium supplemented with 0.1 mg L‑1 ΒΑ and 0.02 mg L‑1 ΝΑΑ was also tested.
Both during initial culture and the subculture, explants responded at high percentages (84-100%) producing shoots in all media.
However, regarding the percentage of explants that produced shoots without hyperhydricity, this was higher in shoot-tip explants (79-98%) than in nodal ones (35-83%), as well as in the control (75-93%) and media containing BA and NAA (80-98%) compared to medium containing only BA (35-79%). More normal shoots per explant (almost double) were produced by nodal explants (1.7-2.1) in comparison to shoot-tip explants (1.0-1.1). During initial culture, the longest shoots (3.0-3.6 cm) were produced in the control, while during subculture, shoot-tip explants cultured on the control or on a medium containing BA and NAA produced longer shoots (2.1-2.4 cm). In conclusion, the use of shoot-tip explants and the addition of ΝΑΑ in the medium limited shoot hyperhydricity, while the use of nodal explants resulted in higher shoot production.
It is one of the most important species of the genus Salvia worldwide, as it is cultivated in many varieties as a medicinal and ornamental plant.
In the present study, the effect of plant growth regulators and explant type on in vitro shoot multiplication of this species was investigated, aiming to the development of a micropropagation protocol that could facilitate breeding and promotion of it for ornamental use.
Shoot-tip and nodal explants excised from in vitro grown seedlings were initially cultured on MS medium without plant growth regulators (control) or supplemented with 0.1 mg L‑1 6-benzyladenine (ΒΑ) and 0.0 or 0.01 mg L‑1 1-naphthaleneacetic acid (NAA). In the following subculture, an additional medium supplemented with 0.1 mg L‑1 ΒΑ and 0.02 mg L‑1 ΝΑΑ was also tested.
Both during initial culture and the subculture, explants responded at high percentages (84-100%) producing shoots in all media.
However, regarding the percentage of explants that produced shoots without hyperhydricity, this was higher in shoot-tip explants (79-98%) than in nodal ones (35-83%), as well as in the control (75-93%) and media containing BA and NAA (80-98%) compared to medium containing only BA (35-79%). More normal shoots per explant (almost double) were produced by nodal explants (1.7-2.1) in comparison to shoot-tip explants (1.0-1.1). During initial culture, the longest shoots (3.0-3.6 cm) were produced in the control, while during subculture, shoot-tip explants cultured on the control or on a medium containing BA and NAA produced longer shoots (2.1-2.4 cm). In conclusion, the use of shoot-tip explants and the addition of ΝΑΑ in the medium limited shoot hyperhydricity, while the use of nodal explants resulted in higher shoot production.
Authors
G. Vlachou, A.N. Martini, M. Papafotiou
Keywords
auxin, cytokinin, hyperhydricity, medicinal and aromatic plant, shoot tip or nodal explant
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Plant-Environment Interactions in Field Systems
- Division Greenhouse and Indoor Production Horticulture
- Division Postharvest and Quality Assurance
- Division Precision Horticulture and Engineering
- Division Horticulture for Human Health
- Division Temperate Tree Nuts
- Division Landscape and Urban Horticulture
- Division Sustaining Horticulture in a Changing World
- Working Group Humulus and Other Cannabaceae
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