Articles
Genome-wide analysis of heat-induced transcriptional regulation by HSFA2 in Rhododendron
Article number
1434_19
Pages
135 – 142
Language
English
Abstract
Heat stress (HS) is the main environmental stress that restricts the growth and development of woody ornamentals of the temperate zone, especially as global warming intensifies. Rhododendron is one of the most important genera of woody flowering plants.
However, the majority of species in this genus are heat-sensitive, which restricts their extensive application.
In previous study, we found that among all transcription factors HSFA2 demonstrates the most robust response to HS in the thermotolerant germplasm Rhododendron hainanense, which can be speculated that HSFA2 may be the dominant factor responsible for the heat tolerance.
To comprehensively elucidate the regulatory network of HSFA2, we conducted a genome-wide analysis by using RNA-seq and DAP-seq.
We found that except HSPs, many other genes involved in heat response and other functions, such as defense response to fungus and response to karrikin, are regulated by HSFA2. And the motifs that can be bound by HSFA2 were analyzed by DAP-seq.
Together, our finding highlights that HSFA2 plays a significant role in many other genes involved in other biological and abiotic processes except heat response in Rhododendron. Based on these findings, we propose a genome-wide regulatory network to elucidate the role of HSFA2 in regulating plant responses to environmental stress in Rhododendron.
However, the majority of species in this genus are heat-sensitive, which restricts their extensive application.
In previous study, we found that among all transcription factors HSFA2 demonstrates the most robust response to HS in the thermotolerant germplasm Rhododendron hainanense, which can be speculated that HSFA2 may be the dominant factor responsible for the heat tolerance.
To comprehensively elucidate the regulatory network of HSFA2, we conducted a genome-wide analysis by using RNA-seq and DAP-seq.
We found that except HSPs, many other genes involved in heat response and other functions, such as defense response to fungus and response to karrikin, are regulated by HSFA2. And the motifs that can be bound by HSFA2 were analyzed by DAP-seq.
Together, our finding highlights that HSFA2 plays a significant role in many other genes involved in other biological and abiotic processes except heat response in Rhododendron. Based on these findings, we propose a genome-wide regulatory network to elucidate the role of HSFA2 in regulating plant responses to environmental stress in Rhododendron.
Authors
Y. Cao, X. Zhang, F. Qu, H. Zhou, Y. Xia, X. Wang
Keywords
regulatory network, heat shock factors, heat stress, DAP-seq, RNA-seq
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