Articles
Rosa1 inserted in RcMYB114 promoter produces red petal coloration in rose through altering its transcription
Article number
1461_2
Pages
7 – 18
Language
English
Abstract
Rose (Rosa sp.) flowers have a rich diversity of colors resulting from the differential accumulation of anthocyanins, flavonols, and carotenoids.
Rose color is an important trait in breeding.
Red petal color in rose is closely associated with anthocyanin biosynthesis.
However, the genetic and molecular determinants of the red-petal trait in roses remain poorly understood.
Here, we report that an R2R3 MYB transcription factor, RcMYB114, is involved in anthocyanin biosynthesis pathway and is responsible for the red petal trait in rose flowers.
During a field survey, we found an interesting red rose (Rosa chinensis ‘Slater’s Crimson China’) specimen that bore both red and white flowers on the same branch.
The flowers had no other obvious differences, having the same number of petals, pistils, stamens, and sepals.
According to the results of RNA-seq analysis we found 125 MYB genes expressed in red flowers and 118 MYB genes expressed in white flowers.
Among these MYB genes, only RcMYB114 showed highly expression in red flowers, but was absent in white flowers.
After detailed research, it was found that a transposable element-like insertion (Rosa1) into RcMYB114, a R2R3-MYB transcription factor’s promoter region activates its transcription, resulting in red petals.
Sequencing, yeast two-hybrid, transient transformation, and promoter activity assays of RcMYB114 collectively confirmed the role of Rosa1 in altering RcMYB114’s transcription and downstream effects on flower color.
Genetic and molecular evidence confirmed that the Rosa1 transposable element-like insertion, which is a novel DNA transposable element, is different from those in other plants and is a reliable molecular marker to screen red-petal roses.
This discovery provides a foundation for the development of new rose cultivars with enhanced color traits.
The unique Rosa1 transposable element-like insertion could be manipulated via genetic engineering for the creation of roses with novel, vibrant petal colors.
Rose color is an important trait in breeding.
Red petal color in rose is closely associated with anthocyanin biosynthesis.
However, the genetic and molecular determinants of the red-petal trait in roses remain poorly understood.
Here, we report that an R2R3 MYB transcription factor, RcMYB114, is involved in anthocyanin biosynthesis pathway and is responsible for the red petal trait in rose flowers.
During a field survey, we found an interesting red rose (Rosa chinensis ‘Slater’s Crimson China’) specimen that bore both red and white flowers on the same branch.
The flowers had no other obvious differences, having the same number of petals, pistils, stamens, and sepals.
According to the results of RNA-seq analysis we found 125 MYB genes expressed in red flowers and 118 MYB genes expressed in white flowers.
Among these MYB genes, only RcMYB114 showed highly expression in red flowers, but was absent in white flowers.
After detailed research, it was found that a transposable element-like insertion (Rosa1) into RcMYB114, a R2R3-MYB transcription factor’s promoter region activates its transcription, resulting in red petals.
Sequencing, yeast two-hybrid, transient transformation, and promoter activity assays of RcMYB114 collectively confirmed the role of Rosa1 in altering RcMYB114’s transcription and downstream effects on flower color.
Genetic and molecular evidence confirmed that the Rosa1 transposable element-like insertion, which is a novel DNA transposable element, is different from those in other plants and is a reliable molecular marker to screen red-petal roses.
This discovery provides a foundation for the development of new rose cultivars with enhanced color traits.
The unique Rosa1 transposable element-like insertion could be manipulated via genetic engineering for the creation of roses with novel, vibrant petal colors.
Authors
M.F. Li, H. Wang, P. Sun, Y.H. Kang, X.Y. Sun, M. Jin, W.M. Jin
Keywords
Rosa1, RcMYB114, transcription, petal color, rose, anthocyanin
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