Articles
Rose (Rosa chinensis) bZIP transcription factors form homo- and heterodimers in response to abiotic stress
Article number
1461_5
Pages
31 – 42
Language
English
Abstract
Rose (Rosa chinensis) is a globally important ornamental crop, whose cultivation efficiency and commercial value are severely compromised by environmental stresses.
Within plant stress response networks, the basic leucine zipper (bZIP) transcription factor family acts as a central regulatory hub, integrating developmental signals and stress adaptation.
Here, we conducted a genome-wide analysis of the bZIP gene in rose and identified 48 RcbZIP family members.
These RcbZIPs phylogenetically clustered into 10 distinct subfamilies which exhibit conserved domain architectures.
Comparative transcriptomic profiling under four abiotic stress conditions (high temperature, salinity, low temperature, and shade) revealed seven network-embedded regulator regulatory genes – RcbZIP7/10/20/28/31/41/45 – exhibiting stress-responsive co-expression patterns.
Promoter cis-element (CE) analysis demonstrated significant enrichment of functional annotated motifs associated with growth regulation (e.g., G-box), hormone signaling (ABRE, TGA-box), and stress adaptation (MYB, STRE, LTR), suggesting a coordinated osmotic-antioxidant-hormone resistance module.
Protein-protein interaction (PPI) predictions and yeast two-hybrid (Y2H) assays further identified RcbZIP40 as a pivotal interaction hub, forming dimeric complexes with multiple RcbZIP proteins.
These findings provide a systematic framework for understanding the evolutionary dynamics and regulatory networks of the bZIP family in roses, offering valuable genetic resources for molecular breeding aimed at enhancing stress resilience.
Within plant stress response networks, the basic leucine zipper (bZIP) transcription factor family acts as a central regulatory hub, integrating developmental signals and stress adaptation.
Here, we conducted a genome-wide analysis of the bZIP gene in rose and identified 48 RcbZIP family members.
These RcbZIPs phylogenetically clustered into 10 distinct subfamilies which exhibit conserved domain architectures.
Comparative transcriptomic profiling under four abiotic stress conditions (high temperature, salinity, low temperature, and shade) revealed seven network-embedded regulator regulatory genes – RcbZIP7/10/20/28/31/41/45 – exhibiting stress-responsive co-expression patterns.
Promoter cis-element (CE) analysis demonstrated significant enrichment of functional annotated motifs associated with growth regulation (e.g., G-box), hormone signaling (ABRE, TGA-box), and stress adaptation (MYB, STRE, LTR), suggesting a coordinated osmotic-antioxidant-hormone resistance module.
Protein-protein interaction (PPI) predictions and yeast two-hybrid (Y2H) assays further identified RcbZIP40 as a pivotal interaction hub, forming dimeric complexes with multiple RcbZIP proteins.
These findings provide a systematic framework for understanding the evolutionary dynamics and regulatory networks of the bZIP family in roses, offering valuable genetic resources for molecular breeding aimed at enhancing stress resilience.
Authors
Y.R. Shi, G.H. Tang, X.Q. Jiang
Keywords
Rosa chinensis, abiotic stress, RcbZIP40, protein interaction
Online Articles (23)