Articles
Rose somatic embryogenic calli developed by the National Institute of Horticultural and Herbal Science and their applications
Article number
1461_23
Pages
203 – 206
Language
English
Abstract
Most plant-derived calluses regenerate into either roots or shoots, but not both.
This type of callus is called somatic embryogenic callus (SEC). It can be considered a type of plant stem cell because it resembles an embryo derived from a reproductive cell and later becomes a seed and grows into a complete plant with shoots and roots.
The National Institute of Horticultural & Herbal Science (NIHHS) under the Rural Development Administration (RDA) in Korea has been developing transgenic rose lines by a genetic transformation technique using the SEC as starting material since 2010. NIHHS successfully obtained SEC from callus formed on segments of in vitro roots of the Korean rose cultivar ‘Sweet Yellow’ in 2007. Since then, the NIHHS has induced the SEC from 9 Korean rose cultivars or breeding lines and has maintained them through subculture.
The NIHHS established protocols for obtaining genetically modified rose plants with the ‘Sweet Yellow’-derived SEC in 2010. Based on this protocol, the NIHHS developed SOD2-transgenic rose lines with enhanced resistance to drought and low temperature stresses in 2014, dsCOPB2-transgenic rose lines with improved resistance to mite in 2017, and VtF3’5’H/RhNHX-transgenic rose lines with changed flower color from white to light purple in 2022. Recently, in vitro rose root-derived SEC have been used as a cosmetic material both in Korea and abroad.
From 2021 to 2022, the NIHHS developed a protocol to obtain callus from petals of the Korean rose cultivar ‘Ice Wing’ and transferred this protocol to a company to use it as a raw material for cosmetics.
These SEC have been maintained by subculture at 4 to 6 weeks’ interval in a culture room at 25±2°C until now.
In addition, it is expected to be used as another unexpected purpose as well as starting material to obtain transgenic rose plant.
This type of callus is called somatic embryogenic callus (SEC). It can be considered a type of plant stem cell because it resembles an embryo derived from a reproductive cell and later becomes a seed and grows into a complete plant with shoots and roots.
The National Institute of Horticultural & Herbal Science (NIHHS) under the Rural Development Administration (RDA) in Korea has been developing transgenic rose lines by a genetic transformation technique using the SEC as starting material since 2010. NIHHS successfully obtained SEC from callus formed on segments of in vitro roots of the Korean rose cultivar ‘Sweet Yellow’ in 2007. Since then, the NIHHS has induced the SEC from 9 Korean rose cultivars or breeding lines and has maintained them through subculture.
The NIHHS established protocols for obtaining genetically modified rose plants with the ‘Sweet Yellow’-derived SEC in 2010. Based on this protocol, the NIHHS developed SOD2-transgenic rose lines with enhanced resistance to drought and low temperature stresses in 2014, dsCOPB2-transgenic rose lines with improved resistance to mite in 2017, and VtF3’5’H/RhNHX-transgenic rose lines with changed flower color from white to light purple in 2022. Recently, in vitro rose root-derived SEC have been used as a cosmetic material both in Korea and abroad.
From 2021 to 2022, the NIHHS developed a protocol to obtain callus from petals of the Korean rose cultivar ‘Ice Wing’ and transferred this protocol to a company to use it as a raw material for cosmetics.
These SEC have been maintained by subculture at 4 to 6 weeks’ interval in a culture room at 25±2°C until now.
In addition, it is expected to be used as another unexpected purpose as well as starting material to obtain transgenic rose plant.
Authors
S.Y. Lee, J.Y. Shin, K.Y. Lee, Y.J. Kim, Y.J. Choi, S.H. Lim
Keywords
cosmetics, dsCOPB2, RhNHX, SOD2, Korean rose cultivars, scent, transgenic plants, VtF3’5’H
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