Articles
THE SELF-INCOMPATIBILITY GENE IN APPLE AND DETERMINATION OF THE S-GENOTYPE OF APPLE CULTIVARS BY PCR
Article number
423_13
Pages
103 – 110
Language
Abstract
Pollen-pistil interactions in apple (Malus X domestica Borkh.) are controlled by a gametophytic self-incompatibility (SI) system that prevents self-pollen from reaching the ovary for fertilization.
Several alleles of the highly polymorphic SI gene (S-gene) in apple have been cloned and characterized.
Comparison of the derived amino acid sequences of S-alleles in apple reveals that the protein structure is composed of alternating conserved and variable sequence regions.
Allele-specific PCR primers, designed to anneal within the variable sequence domains, were used to analyse the presence of the corresponding S-alleles in genomic DNA from various cultivars.
Here we report on recent data on the S-genotypes of old and current apple cultivars, determined by PCR typing of the S-alleles.
Several alleles of the highly polymorphic SI gene (S-gene) in apple have been cloned and characterized.
Comparison of the derived amino acid sequences of S-alleles in apple reveals that the protein structure is composed of alternating conserved and variable sequence regions.
Allele-specific PCR primers, designed to anneal within the variable sequence domains, were used to analyse the presence of the corresponding S-alleles in genomic DNA from various cultivars.
Here we report on recent data on the S-genotypes of old and current apple cultivars, determined by PCR typing of the S-alleles.
Publication
Authors
W. Broothaerts, L. Verdoodt, J. Keulemans, G.A. Janssens, W.F. Broekaert
Keywords
Allele-specific PCR, Malus X domestica, pistil, pollination, ribonuclease, S-allele, S-gene
Online Articles (32)