Articles
IN VITRO SCREENING OF COLD RESISTANT STRAWBERRY CULTIVARS AND SEEDLINGS
Article number
439_32
Pages
221 – 226
Language
Abstract
Cold resistance of plants and proliferating structures in vitro have been studied within nine strawberry cultivars and seedlings of 4 combinations.
Cultivars, known for their characters, were used as model in order to identify the possibilities for selection of cold-resistant seedlings in vitro. Microplants and proliferating structures were hardened for maximum 35 days at 2±0.5°C, frozen for 1–8 days at -8°C, -9°C, -10°C, -11°C, -12°C, -14°C in vitro in the media Murashige and Skoog with 1.5, 3, 6 or 9% sucrose.
Explants were thawed at 2±0.5°C for 1–3 days.
The degree of cold injury was evaluated according to 6-point scale (0–5). The best temperatures for freezing in vitro for differentiation of cultivars for cold resistance were -9°C to -11°C. Explants were more viable, whem time of hardening was longer.
Viability of plants also depends on sucrose amount in the media.
Cultivars, known for their characters, were used as model in order to identify the possibilities for selection of cold-resistant seedlings in vitro. Microplants and proliferating structures were hardened for maximum 35 days at 2±0.5°C, frozen for 1–8 days at -8°C, -9°C, -10°C, -11°C, -12°C, -14°C in vitro in the media Murashige and Skoog with 1.5, 3, 6 or 9% sucrose.
Explants were thawed at 2±0.5°C for 1–3 days.
The degree of cold injury was evaluated according to 6-point scale (0–5). The best temperatures for freezing in vitro for differentiation of cultivars for cold resistance were -9°C to -11°C. Explants were more viable, whem time of hardening was longer.
Viability of plants also depends on sucrose amount in the media.
Publication
Authors
R. Rugienius, V. Stanys
Keywords
Fragaria x ananassa, frost tolerance
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