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Articles

MERISTEM CULTURE OF AZALEAS (RHODODENDRON SIMSII)

Article number

78_26

Pages

203 – 208

Language

Abstract

Normal shoot development can be induced in tip meristem explants by 2–5 ppm benzylaminopurine (BAP), when using the medium after Murashige and Skoog (1962) at 1/10 strength with 2% sucrose and without indoleacetic acid (IAA).

After shoot differentiation 2 ppm IAA in connection with active coal (0.25%) and in absence of BAP induce root development.
IAA supplied to the medium at the beginning of the meristem culture causes fast callus growth.
Rooted plants can be obtained about 90–120 days after preparation of the meristems.
The best environmental conditions were found at a constant temperature of 25°C and a light intensity of 3000 Lux 16 hours per day.

Publication

Symposium on Tissue Culture for Horticultural Purposes

Authors

W. Preil, M. Engelhardt

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1977.78.26

Online Articles (56)

2. PLANT CELL AND ORGAN CULTURES AS HORTICULTURAL PRACTICES

T. Murashige

3. THE DEVELOPMENT AND ESTABLISHMENT OF A COMMERCIAL TISSUE CULTURE LABORATORY

D.P. Holdgate | J.S. Aynsley

4. REDUCING TISSUE CULTURE COSTS FOR COMMERCIAL PROPAGATION

R.A. de Fossard | R.A. Bourne

5. TISSUE CULTURE METHODS AND GERMPLASM STORAGE IN POTATO

R.J. Westcott | G.G. Henshaw | B.W.W. Grout | W.M. Roca

6. HORTICULTURAL AND PHYSIOLOGICAL ASPECTS OF CITRUS BUD CULTURE

A. Altman | R. Goren

7. CHANGES IN ISOPEROXIDASES DURING DIFFERENTIATION OF CULTURED TOBACCO EPIDERMAL LAYERS

Th. Gaspar | M. Tran Thanh Van | T.A. Thorpe

8. BIOCHEMICAL PROPERTIES OF TISSUE CULTURES FROM DIFFERENT ORGANS OF ACTINIDIA CHINENSIS

A.-M. Hirsch | D. Bligny | B.K. Tripathi

9. PORPHYRIN METABOLISM IN PEANUT CELLS CULTURED IN SUCROSE CONTAINING MEDIUM

R.B. van Huystee

10. IN VITRO TECHNIQUES AS AN EFFICIENT TOOL IN ASPARAGUS BREEDING

C. Dore

11. USE OF MICROPROPAGATION AND TISSUE CULTURE TO INVESTIGATE GENETIC DISORDERS IN ALMOND CULTIVARS

D.E. Kester | L. Tabachnik | J. Negueroles

12. IRRADIATION STUDIES ON HIGHER PLANT ORGAN

J. Mousseau

13. EFFECT OF CHLOROGENIC ACIDS ON THE GROWTH OF EXCISED YOUNG STEM SEGMENTS OF PRUNUS AVIUM

W. Feucht | C.S. Johal

14. THE GROWTH POTENTIAL OF OLIVE FRUIT MESOCARP IN VITRO (OLEA EUROPEA)

S. Lavee

15. ORGANIC ACIDS AND POTASSIUM REQUIREMENT OF CALLUS TISSUE DERIVED FROM ORANGE PEEL

O. Reuveni | A. Bar-Akiva | J. Sagiv

16. EXPERIMENTAL DATA FOR A STRATEGY OF THE IMPROVEMENT OF THE SHOOT – FORMING CAPACITY IN VITRO

C. Bigot | S. Ohki | J. Mousseau

17. REGENERATION OF PLANTS FROM CALLUS OF POTATO TUBERS

M. Bragdø-Aas

18. IN VITRO STUDIES ON ORGANOGENESIS AND GROWTH IN ALLIUM CEPA TISSUE CULTURES

D.I. Dunstan | K.C. Short

19. FACTORS INFLUENCING THE REGENERATION OF EUCALYPTUS GRANDIS BY ORGAN CULTURE

R. Durand-Cresswell | C. Nitsch

20. THE ORGAN CULTURE OF NODES OF FOUR SPECIES OF EUCALYPTUS

R.A. de Fossard | Pamela K. Barker | R.A. Bourne

21. MORPHOGENESIS AND PLANT REGENERATION FROM CULTURED ORGAN FRAGMENTS OF CYCLAMEN PERSICUM

T. Geier

22. INFLUENCE OF SEVERAL GROWTH REGULATORS AND AMINO ACIDS ON IN VITRO ORGANOGENESIS OF TORENIA AND DAUCUS

H. Kamada | H. Harada

23. DIFFERENTIATION OF CULTIVATED LEAF DISCS OF PRUNUS MAHALEB

C.M. Hedtrich

24. EFFECT OF GALACTOSE AND GALACTOSE CONTAINING SUGARS ON EMBRYOGENESIS IN CITRUS CALLUS

J. Kochba | P. Spiegel-Roy | S. Saad | H. Neuman

25. SOME FACTORS AFFECTING MULTIPLE BUD FORMATION OF STRAWBERRY (X FRAGARIA ANANASSA DUCHESNE) IN VITRO

E.C.M. Lee | R.A. de Fossard

26. PLANTLET FORMATION FROM EXCISED BULB SCALE SEGMENTS OF NERINE

R.L.M. Pierik | B.J. Ippel

27. MERISTEM CULTURE OF AZALEAS (RHODODENDRON SIMSII)

W. Preil | M. Engelhardt

28. CLONAL MULTIPLICATION OF AZALEAS THROUGH TISSUE CULTURE

S.S. Ma | S.O. Wang

29. ADVENTITIOUS ORGAN FORMATION AND SOMATIC EMBRYOGENESIS IN CALLUS OF ASPARAGUS AND IRIS AND ITS POSSIBLE APPLICATION

G. Reuther

30. THE EFFECT OF SUBCULTURE INTERVAL ON ORGANOGENESIS IN CALLUS CULTURES OF CITRUS SINENSIS

J. Button | F.H.J. Rijkenberg

31. CELLULAR TOTIPOTENCY IN CHRYSANTHEMUM TISSUES CULTURED IN VITRO

R.S. Sangwan | H. Harada

32. THE INITIATION, ROOTING AND ESTABLISHMENT OF CORTICAL BUDS IN CAULIFLOWER

D.S. Trimboli | N. Prakash | R.A. de Fossard

33. ORGANOGENESIS IN CULTURES OF SAINTPAULIA IONANTHA

A.M. Vazquez | M.R. Davey | K.C. Short

34. MORPHOGENESIS IN VITRO OF PINUS AND PICEA

K.J. Webb | H.E. Street

35. INDUCTION OF ANDROGENESIS IN VITRO IN MALUS DOMESTICA

E. Milewska-Pawliczuk | B. Kubicki

36. CULTURE OF COCONUT AND DATE PALM TISSUE WITH A VIEW TO VEGETATIVE PROPAGATION

C.J. Eeuwens | J. Blake

37. GROWTH AND DEVELOPMENT OF ASPARAGUS PLUMOSUS SHOOT TIPS IN VITRO

A. Fonnesbech | M. Fonnesbech | N. Bredmose

38. PRACTICAL ASPECTS OF THE PROPAGATION OF CAULIFLOWER BY MERISTEM CULTURE

B.W.W. Grout | P. Crisp

39. PROPAGATION OF HOOP PINE (ARAUCARIA CUNNINGHAMII AIT.) BY ORGAN CULTURE

R.J. Haines | R.A. de Fossard

40. IN VITRO PROPAGATION OF SOME MEMBERS OF THE LILIACEAE, IRIDACEAE AND AMARYLLIDACEAE

G. Hussey

41. IN VITRO PROPAGATION OF SOME TILLANDSIOIDEAE (BROMELIACEAE)

O. Mekers

42. AUXIN: CYTOKININ INTERACTIONS IN THE IN VITRO MICRO-PROPAGATION OF STRAWBERRY PLANTS

D.J. James | B. Newton

43. MAINTAINED CULTURE OF MULTIPLE PLANTLETS FROM CARNATION SHOOT TIPS

S. Jelaska | R. Sutina

44. FACTORS AFFECTING THE COLONY FORMING ABILITY OF FREE CELLS OF HAPLOPAPPUS GRACILIS (NUTT.) GRAY

P.A.Th.J. Werry | K.M. Stoffelsen

45. MASS PROPAGATION OF FICUS LYRATA

P. Debergh | J. De Wael

46. REGENERATION OF ENTIRE PLANTS OF PEPEROMIA SCANDENS RUIZ. FROM DIFFERENT PARTS OF LEAVES IN VITRO

K. Kukulczanka | K. Klimaszewska | H. Pluta

47. NURSERY BEHAVIOUR OF FRUIT TREES PROPAGATED BY “IN VITRO” CULTURE

Ph. Boxus | M. Quoirin

48. THERMOTHERAPY AND MERISTEM-TIP CULTURE OF SOME ORNAMENTAL PLANTS

D. Cohen

49. LOCALIZATION OF VIRUSES IN APICAL MERISTEM AND PRODUCTION OF VIRUS-FREE PLANTS BY MEANS OF MERISTEM AND TISSUE CULTURE

K. Mori | D. Hosokawa

50. CLONALE PROPAGATION OF PELARGONIUM X HORTORUM AND PELARGONIUM X PELTATUM FROM MERISTEM CULTURE FOR DISEASE FREE CUTTINGS.

G. Beauchesne | J. Albouy | J.C. Morand | J. Daguenet

51. IN VITRO CULTURE OF SHOOT TIPS OF PELARGONIUM SPECIES

R. Theiler

52. MERISTEM-TIP CULTURE AND VEGETATIVE PROPAGATION IN POTATO

F. Marani | A. Pisi

53. TISSUE CULTURE TECHNIQUES USED IN SPAIN TO RECOVER VIRUS-FREE CITRUS PLANTS

L. Navarro | J. Juarez

54. IMPROVED MEDIA FOR IN VITRO CULTURE OF PRUNUS SP.

M. Quoirin | P. Lepoivre

55. PRODUCTION OF BEAN MESOPHYLL PROTOPLASTS AND INOCULATION WITH TOBACCO NECROSIS VIRUS OR TOBACCO MOSAIC VIRUS

D.H. Wieringa-Brants

56. RAPID CLONAL PROPAGATION OF PATHOGEN-FREE PELARGONIUM PLANTS STARTING FROM SHOOT TIPS AND APICAL MERISTEMS

P. Debergh | L. Maene