Articles
MORPHOGENESIS IN VITRO OF PINUS AND PICEA
Article number
78_33
Pages
259 – 270
Language
Abstract
Cultures have been initiated from embryos and seedlings and from shoot meristems and needles of 2 year old seedlings of Pinus contorta and Picea sitchensis. Excised embryos of P. contorta and P. sitchensis and 2 year old shoot meristems of P. itchensis form adventitious buds under defined cultural conditions.
Alterations in the hormonal milieu result in either the direct formation of buds on the original explant or the initiation of a callus with associated buds.
Subculture of the callus with buds onto the same initiation medium results in further bud formation, whereas transfer to a low salt, hormone-free medium promotes the outgrowth of shoots from the preformed buds.
As the shoots extend, they may be individually dissected out and rooted by traditional non-sterile sylvicultural techniques.
Alterations in the hormonal milieu result in either the direct formation of buds on the original explant or the initiation of a callus with associated buds.
Subculture of the callus with buds onto the same initiation medium results in further bud formation, whereas transfer to a low salt, hormone-free medium promotes the outgrowth of shoots from the preformed buds.
As the shoots extend, they may be individually dissected out and rooted by traditional non-sterile sylvicultural techniques.
Authors
K.J. Webb, H.E. Street
Keywords
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