Articles
USE OF ANTIOXIDANTS FOR ENHANCING FLAX MULTIPLICATION RATE IN TISSUE CULTURE
Article number
812_15
Pages
147 – 152
Language
English
Abstract
The aim of investigation was to develop a tissue culture approach that includes combination of a seed sterilisation method, the use of silver nitrate and antioxidants during different stages of calli induction, and shoot regeneration from flax accessions with low regeneration capacity.
All used flax varieties (Vega 2, Lirina, and Rigaer B) were excellent for calli culture formation (100% of explants form calli). Addition of AgNO3 to the seed germination medium promoted calli culture formation.
Development of plants-regenerants was depended on the used 2,4-D concentration in calli induction medium, antioxidant, explant type (stem segments, leaves) and genotype but was not related to the calli weight.
Antioxidants increased calli regeneration capacity.
Plants-regenerants were obtained only from stem calli.
All used flax varieties (Vega 2, Lirina, and Rigaer B) were excellent for calli culture formation (100% of explants form calli). Addition of AgNO3 to the seed germination medium promoted calli culture formation.
Development of plants-regenerants was depended on the used 2,4-D concentration in calli induction medium, antioxidant, explant type (stem segments, leaves) and genotype but was not related to the calli weight.
Antioxidants increased calli regeneration capacity.
Plants-regenerants were obtained only from stem calli.
Publication
Authors
D. Grauda, A. Miķelsone, I. Rashal
Keywords
calli culture, ambiol, AV-153, ascorbic acid, triovit, regeneration
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