Articles
DEVELOPMENT, MATURATION AND GERMINATION OF HORSE CHESTNUT SOMATIC EMBRYOS
Article number
812_41
Pages
301 – 304
Language
English
Abstract
Somatic embryogenesis could represent a way to clonally propagate mature elite trees of horse chestnut.
However, while a number of protocols describe successfully the induction and proliferation of somatic embryos a low frequency of embryos converted successfully into plants.
The objective of this research was to identify a maturation medium that could contribute to the outcome of more efficient germination results.
Therefore, in a first approach the effects of sucrose 3 and 6%, and maltose 3%, were evaluated on somatic embryo maturation, in terms of subsequent somatic embryo germination.
Carbon source and concentration had a marked influence on maturation, and subsequent germination of horse chestnut somatic embryos, with sucrose 6% promoting the highest frequency of germinated embryos larger than 1 cm.
However, while a number of protocols describe successfully the induction and proliferation of somatic embryos a low frequency of embryos converted successfully into plants.
The objective of this research was to identify a maturation medium that could contribute to the outcome of more efficient germination results.
Therefore, in a first approach the effects of sucrose 3 and 6%, and maltose 3%, were evaluated on somatic embryo maturation, in terms of subsequent somatic embryo germination.
Carbon source and concentration had a marked influence on maturation, and subsequent germination of horse chestnut somatic embryos, with sucrose 6% promoting the highest frequency of germinated embryos larger than 1 cm.
Publication
Authors
V. Troch, N. Vermeir, S. Werbrouck, M.-C. Van Labeke
Keywords
Aesculus hippocastanum, somatic embryogenesis, embryo maturation, osmoticum
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