Articles
COMPARISON OF IN VITRO AND TRADITIONAL PROPAGATION METHODS OF RHUBARB (RHEUM RHABARBARUM) ACCORDING TO MORPHOLOGICAL FEATURES AND YIELD
Article number
812_35
Pages
265 – 270
Language
English
Abstract
Rhubarb (Rheum rhabarbarum) is one of the first vegetables harvested in Latvia from May till June.
Mostly it is propagated by mother plant division.
In some cases it is multiplied by seeds, but as it is known that the progeny in this case is not homogeneous.
Micropropagation has been used successfully for rhubarb propagation for several decades.
Till now it has not been applied for Latvian genotypes.
Rhubarb was propagated in vitro for two years to obtain homogeneous planting material.
Three media compositions were used to obtain the highest proliferation rate: M&S mineral salt medium, vitamins 1 mg L-1; inozitol 100 mg L-1; 6-benzilaminopurine (BAP) 1, 1.5 and 2 mg L-1; indole-3-butyric acid (IBA) 1 mg L-1 and in one case gibberellic acid (GA3) 0.5 mg L-1, sucrose 20 g L-1, agar 8 g L-1, pH 5.5. Most of the genotypes had good proliferation in the MS media with BAP 1.5 mg L-1 and IBA 0.7 mg L-1. Micropropagated and traditionally propagated plants were planted in the field to evaluate their morphological and yield features.
The following morphological features were analysed: leaf number, leaf size, petiole length, overwintering.
Yield was evaluated for one year (second year of vegetation). The results of the first two seasons show that micropropagated plants and seedlings were better developed and yielded than plants obtained by mother plants dividing.
Mostly it is propagated by mother plant division.
In some cases it is multiplied by seeds, but as it is known that the progeny in this case is not homogeneous.
Micropropagation has been used successfully for rhubarb propagation for several decades.
Till now it has not been applied for Latvian genotypes.
Rhubarb was propagated in vitro for two years to obtain homogeneous planting material.
Three media compositions were used to obtain the highest proliferation rate: M&S mineral salt medium, vitamins 1 mg L-1; inozitol 100 mg L-1; 6-benzilaminopurine (BAP) 1, 1.5 and 2 mg L-1; indole-3-butyric acid (IBA) 1 mg L-1 and in one case gibberellic acid (GA3) 0.5 mg L-1, sucrose 20 g L-1, agar 8 g L-1, pH 5.5. Most of the genotypes had good proliferation in the MS media with BAP 1.5 mg L-1 and IBA 0.7 mg L-1. Micropropagated and traditionally propagated plants were planted in the field to evaluate their morphological and yield features.
The following morphological features were analysed: leaf number, leaf size, petiole length, overwintering.
Yield was evaluated for one year (second year of vegetation). The results of the first two seasons show that micropropagated plants and seedlings were better developed and yielded than plants obtained by mother plants dividing.
Publication
Authors
L. Lepse
Keywords
micropropagation, field performance, overwintering, yielding
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