Articles
TUBER FORMATION OF GLORIOSA SUPERBA USING STEM SECTIONS OF BRANCHES UNDER CULTIVATION
Article number
812_32
Pages
245 – 250
Language
English
Abstract
We examined the efficient methods for tuber formation of Gloriosa superba using stem branches under cultivation in green house.
Firstly, we studied the effect of longitudinal division of stem for explants derived from branches under cultivation.
The stem explants did not affect having longitudinal division treatment or not, and had similar organogenesis.
The number of tuber formation increased by treatment with higher concentrations of sucrose and showed maximum at sucrose 80 g L-1 + TDZ (thidiazuron) 3 μM. The number of tuber formation from decapitated stem explants (DS) after decapitation showed more than the double (8.9/explants) of the apical stem explants (AS). In trehalose treatment, the number of tuber formation of the AS was approximately similar to that of sucrose treatment.
In LS explants, the number of tuber formation showed maximum (9.0) by treatment with trehalose 40 g L-1 + TDZ 3 μM and decreased with treatment of trehalose 80 g L-1 + TDZ 3 μM. It becomes clear that LS have higher ability for tuber formation.
Combining TDZ 3 μM with 80 g L-1 sucrose or 40 g L-1 trehalose are highly effective for multiple tuber formation in stem cultures of G. superb.
Firstly, we studied the effect of longitudinal division of stem for explants derived from branches under cultivation.
The stem explants did not affect having longitudinal division treatment or not, and had similar organogenesis.
The number of tuber formation increased by treatment with higher concentrations of sucrose and showed maximum at sucrose 80 g L-1 + TDZ (thidiazuron) 3 μM. The number of tuber formation from decapitated stem explants (DS) after decapitation showed more than the double (8.9/explants) of the apical stem explants (AS). In trehalose treatment, the number of tuber formation of the AS was approximately similar to that of sucrose treatment.
In LS explants, the number of tuber formation showed maximum (9.0) by treatment with trehalose 40 g L-1 + TDZ 3 μM and decreased with treatment of trehalose 80 g L-1 + TDZ 3 μM. It becomes clear that LS have higher ability for tuber formation.
Combining TDZ 3 μM with 80 g L-1 sucrose or 40 g L-1 trehalose are highly effective for multiple tuber formation in stem cultures of G. superb.
Publication
Authors
K. Shimasaki, K. Sakuma, Y. Nishimura
Keywords
tuber formation (PLB), stem culture, micropropagation, sucrose, trehalose, thidiazuron (TDZ)
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