Articles
BACTERIAL CONTAMINATION IN ILEX DUMOSA (AQUIFOLIACEAE) CULTURES: ANTIBIOTIC TREATMENT
Article number
812_6
Pages
97 – 102
Language
English
Abstract
A range of commercially antibiotics were tested on nodal segments-culture of Ilex dumosa for their ability to control bacterial (Gram negative) contamination.
Thirteen treatment with 4 antibiotic were tested: streptomycin (200 mg.ml-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mgml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 μM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 μmolm-2s-1 and 14 h photoperiod.
After 30 days of culture various antibiotics controlled the proliferation of bacteria.
The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average.
Rooting of regenerated shoots produced plants that were successfully transferred in pots.
Thirteen treatment with 4 antibiotic were tested: streptomycin (200 mg.ml-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mgml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 μM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 μmolm-2s-1 and 14 h photoperiod.
After 30 days of culture various antibiotics controlled the proliferation of bacteria.
The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average.
Rooting of regenerated shoots produced plants that were successfully transferred in pots.
Publication
Authors
C. Luna, M. Collavino, P. Sansberro, L. Mroginski
Keywords
immersion temporal system, RITA, plant regeneration
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