Articles
MICROPROPAGATION OF MALUS SIEBOLDII HYBRIDS RESISTANT TO APPLE PROLIFERATION DISEASE
Article number
839_1
Pages
35 – 41
Language
English
Abstract
Natural resistance to apple proliferation (AP) disease was found in apomictic Malus sieboldii-derived genotypes which can be used as rootstocks for apple.
Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures.
Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes.
Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 µM IBA, 4.44 µM BAP and 0.28 µM GA3) and vitamins (MS modified for thiamine at 2.96 µM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes.
The MS medium yielded in general the highest proliferation rates and the best shoot growth.
A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source.
By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 µM IBA either in liquid or agar-solidified medium.
Three classes of genotypes with low, medium and high rooting efficiency were found.
The acclimatisation method used yielded survival rates between 90-100% for most of the genotypes.
Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures.
Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes.
Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 µM IBA, 4.44 µM BAP and 0.28 µM GA3) and vitamins (MS modified for thiamine at 2.96 µM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes.
The MS medium yielded in general the highest proliferation rates and the best shoot growth.
A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source.
By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 µM IBA either in liquid or agar-solidified medium.
Three classes of genotypes with low, medium and high rooting efficiency were found.
The acclimatisation method used yielded survival rates between 90-100% for most of the genotypes.
Authors
A.M. Ciccotti, C. Bisognin, I. Battocletti, A. Salvadori, M. Herdemertens, M. Wallbraun, W. Jarausch
Keywords
apple rootstock, Candidatus Phytoplasma mali, in vitro rooting, plant tissue culture, culture media
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