Articles
TRANSFERABILITY OF MALUS × DOMESTICA MICROSATELLITE MARKERS TO OTHER SPECIES AND GENERA OF THE MALOIDEAE SUBFAMILY
Article number
839_78
Pages
567 – 574
Language
English
Abstract
To check the true hybrid character of seedlings, resulting from interspecific and intergeneric hybridizations in Malus × domestica, we have chosen macrosatellites (SSRs) as molecular markers since SSR loci are highly conserved among species and genera and because SSR primer information is available in cultivated apple.
To study the transferability of apple SSRs to other species or genera of potential breeding parents within the Maloideae we screened 147 accessions of the Maloideae.
These 147 accession covered 10 genera, with several representative species within each genus: Amelanchier (9 accessions), Aronia (5), Chaenomeles (8), Cotoneaster (11), Crataegus (9), Cydonia (11), Malus (20), Mespilus (10), Pyrus (47) and Sorbus (16). In total we tested 31 Malus × domestica SSR loci on each accession.
We considered a marker as transferable when a successful amplification was obtained in at least 75% of the tested accessions.
The transferability from cultivated apple to other genera and Malus species varied between 58% (Pyrus) and 94% (Malus). The mean number of alleles per (transferable) locus ranged from 1.9 (Cydonia) and 2.2 (Mespilus) to 11.5 (Malus) and 12.5 (Pyrus). The low allele diversity of a locus of Cydonia and Mespilus reflects most likely their apomictic behavior.
Considering the transferability of the primer combinations from M. domestica to other genera substantial differences were found among microsatellites: from 0% to 100%, but for most of the primer combinations the transferability of a microsatellite marker was higher than 70%. The highest number of alleles over all species and genera of a specific locus was 62, the lowest only 5. In the latter case the primer combination of this locus amplified just one allele in most species.
To study the transferability of apple SSRs to other species or genera of potential breeding parents within the Maloideae we screened 147 accessions of the Maloideae.
These 147 accession covered 10 genera, with several representative species within each genus: Amelanchier (9 accessions), Aronia (5), Chaenomeles (8), Cotoneaster (11), Crataegus (9), Cydonia (11), Malus (20), Mespilus (10), Pyrus (47) and Sorbus (16). In total we tested 31 Malus × domestica SSR loci on each accession.
We considered a marker as transferable when a successful amplification was obtained in at least 75% of the tested accessions.
The transferability from cultivated apple to other genera and Malus species varied between 58% (Pyrus) and 94% (Malus). The mean number of alleles per (transferable) locus ranged from 1.9 (Cydonia) and 2.2 (Mespilus) to 11.5 (Malus) and 12.5 (Pyrus). The low allele diversity of a locus of Cydonia and Mespilus reflects most likely their apomictic behavior.
Considering the transferability of the primer combinations from M. domestica to other genera substantial differences were found among microsatellites: from 0% to 100%, but for most of the primer combinations the transferability of a microsatellite marker was higher than 70%. The highest number of alleles over all species and genera of a specific locus was 62, the lowest only 5. In the latter case the primer combination of this locus amplified just one allele in most species.
Authors
L. Vanwynsberghe, L. Decq, J. Keulemans
Keywords
Malus × domestica, macrosatellites, interspecific transferability, intergeneric transferability, SSR polymorphism, allelic diversity, phylogenetic relationships
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