Home > I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008 > CHARACTERIZATION OF TRANSGENIC APPLES EXPRESSING THE HrpN GENE

Articles

CHARACTERIZATION OF TRANSGENIC APPLES EXPRESSING THE HrpN GENE

Article number

839_87

Pages

637 – 644

Language

English

Abstract

Fire blight is the major bacterial disease of Maloideae caused by the necrogenic bacterium Erwinia amylovora, which pathogenicity requires a functional type III protein secretion system (TTSS). The protein harpin N_Ea is secreted through the TTSS and targeted to the intercellular spaces of plant tissues where it acts as an elicitor of plant defense reactions.
In order to create novel mechanisms for fire blight resistance in apple, we have generated transgenic apples expressing the hrpN gene from E. amylovora under the control of the constitutive CaMV35S or the pathogen-inducible tobacco Str246C promoters, with or without the tobacco PR1 gene secretion signal peptide.
The characterization of these harpin N_Ea transgenic apples was carried out by: (1) quantification of the constitutive and induced expression of the transgene (by Q-RT-PCR); (2) in planta localization of the harpin N_Ea protein; (3) evaluation of fire blight resistance after greenhouse inoculation; (4) targeted transcriptomic analysis to identify defenses induced by the presence of the transgene.

Publication

I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008

Authors

E. Vergne, S. Sourice, F. Dupuis, K. Loridon, T. Dugé de Bernonville, M.N. Brisset, E. Chevreau

Keywords

fire blight, Malus × domestica Borkh., effector, defense

Full text

https://doi.org/10.17660/ActaHortic.2009.839.87

Online Articles (99)

1. MICROPROPAGATION OF MALUS SIEBOLDII HYBRIDS RESISTANT TO APPLE PROLIFERATION DISEASE

2. MICROPROPAGATION, ROOTING AND ACCLIMATIZATION OF BLACKBERRY ‘AGAVAM’

L. Lepse | V. Laugale

3. GENOTYPIC DIFFERENCES IN SHOOT MULTIPLICATION AMONG FIVE CITRUS ROOTSTOCKS IN VITRO

S. Sen | V. Dhawan

4. CITRUS LIMON MICROPROPAGATION: EFFECT OF DIFFERENT PHYTOHORMONES ON MULTIPLICATION AND ROOTING

O. Pérez-Tornero | C. Tallón | I. Porras

5. MICROPROPAGATION OF ‘TROYER’ CITRANGE [PONCIRUS TRIFOLIATA (L.) RAT. × C. SINENSIS (L.) OSBECK]

S. Sen | V. Dhawan

6. PROPAGATION OF SOME DATE PALM CULTIVARS BY USING TISSUE CULTURE METHODS

I. Pinker | Y.M. Ibraheem | M.H. Böhme

7. THE INFLUENCE OF IMIDAZOLE FUNGICIDES ON MULTIPLICATION IN VITRO OF LOW VIGOROUS PEAR AND CHERRY ROOTSTOCKS

DJ. Ružić | T. Vujović | R. Cerović | M. Kuzmanović

8. IN VITRO PROLIFERATION OF NEWLY BRED CZECH PEAR CULTIVARS

J. Sedlak | F. Paprstein

9. PHOTOAUTOTROPHIC MICROPROPAGATION FOR COST-EFFECTIVE AND SUCCESSFUL CLONAL MULTIPLICATION OF WOODY FRUIT CROPS

K.J. Pawan | V.A. Kumar | S. Shah | A. Kumar

10. IN VITRO PROPAGATION OF WILD EUROPEAN PLUM (PRUNUS ×DOMESTICA L.), A RARE AND ENDANGERED SPECIES

11. DEVELOPMENT OF A MICROPROPAGATION PROTOCOL FOR THE ROMANIAN QUINCE (CYDONIA OBLONGA) CULTIVAR ‘AURII’ AND ROOTSTOCKS ‘BN70’ AND ‘A TYPE’

A. Giorgota | S. Preda | M. Isac | M. Tulvinschi

12. MICROPROPAGATION OF SELECTED TREES OF ARBUTUS UNEDO L. THROUGH AXILLARY SHOOT PROLIFERATION AND SOMATIC EMBRYOGENESIS

F. Gomes | M.L. Lopes | T. Santos | J.M. Canhoto

13. IMPROVED MICROPROPAGATION OF WALNUT (JUGLANS REGIA L.) ON MEDIA OPTIMIZED FOR GROWTH BASED UPON MINERAL CONTENT OF WALNUT SEED

K. Vahdati | E. Najafian Ashrafi | H. Ebrahimzadeh | M. Mirmasoumi

14. IN VITRO PROPAGATION OF SOME GRAPE ROOTSTOCKS

S.Z. El-Agamy | T.K. El-Mahdy | A.A. Mohamed

15. IN VITRO PROPAGATION OF ZIZYPHUS SPINA-CHRISTI L.

S.Z. El-Agamy | T.K. El-Mahdy | D.SH. El-Kasas

16. REGENERATION AND FIELD EVALUATION OF BANANA (MUSA ACUMINATA, AAA GROUP) PLANTS FROM PROLIFERATING INFLORESCENCE-DERIVED EMBRYOGENIC SUSPENSION CULTURES

J.A. Cabrera-García | J. Cabrera-Cabrera | J.B. Pérez-Hernández | M.T. Cruz-Bacallado

17. FACTORS AFFECTING SOMATIC EMBRYOGENESIS INDUCTION AND DEVELOPMENT IN FEIJOA SELLOWIANA BERG.

J.M. Canhoto | S.I. Correia | C.I. Marques

18. SOMATIC EMBRYOGENESIS IN TAMARILLO (CYPHOMANDRA BETACEA): RECENT ADVANCES

S.I. Correia | M.L. Lopes | J.M. Canhoto

19. EFFECT OF CARBOHYDRATE SOURCE AND POLYETHYLENE GLYCOL ON MATURATION AND GERMINATION OF SOMATIC EMBRYOS IN WALNUT (JUGLANS REGIA L.)

H. Bahrami Sirmandi | K. Vahdati

20. IN VITRO GERMINATION OF STONELESS SEEDS AND ISOLATED EMBRYOS OF 14 SICILIAN CULTIVARS OF OLEA EUROPAEA L.

M.A. Germanà | G. Patricolo | B. Chiancone

21. IN VITRO GERMINATION AND SEEDLING DEVELOPMENT OF CAPER (CAPPARIS SPINOSA L.) MATURE SEEDS

M.A. Germanà | B. Chiancone

22. INTRODUCING NEW GENES INTO ‘THOMPSON SEEDLESS’ GRAPES

S.Z. El-Agamy | H.A.A. Galil | M.A.M. Elsysy

23. EFFICIENT SHOOT REGENERATION SYSTEM OF PEAR ROOTSTOCK OHF 333 (PYRUS COMMUNIS L.) LEAVES

L.R. Nacheva | P.S. Gercheva | V.T. Dzhuvinov

24. IMPACT OF EXOGENOUS SUCROSE, RAFFINOSE AND PROLINE ON COLD ACCLIMATION OF STRAWBERRY IN VITRO

V. Lukoševičiūtė | R. Rugienius | A. Sasnauskas | V. Stanys | C. Bobinas

25. COMMERCIALLY FEASIBLE PROTOCOL FOR ROOTING AND ACCLIMATIZATION OF MICROPROPAGATED APPLE ROOTSTOCKS

M. Modgil | T. Sharma | M. Thakur

26. IN VITRO ROOTING IMPROVEMENT OF ADULT PISTACHIO, PISTACIA VERA L. ‘ATLI’

E. Tilkat | A. Onay | Y. Ozden Tokatli

27. ROOTING AND MULTIPLICATION ABILITY OF PERSIAN WALNUT AS INFLUENCED BY MOTHERSTOCK VIGOR AND PRECOCITY

K. Vahdati | M. Mirmasoumi | R. Rezaee

28. CRYOPRESERVATION OF PAPAYA (CARICA PAPAYA L.) SEED: OVERCOMING DORMANCY AND OPTIMIZING SEED DESICCATION AND STORAGE CONDITIONS

S.E. Ashmore | R.A. Drew | C. O'Brien | A. Parisi

29. ADVANCES IN THE CRYOPRESERVATION OF FRUIT PLANT GERMPLASM AT THE CNR-IVALSA INSTITUTE OF FLORENCE

M. Lambardi | C. Benelli | A. De Carlo | A. Previati

30. APPLICATION OF CRYOPRESERVATION FOR PISTACHIO GERMPLASM CONSERVATION

Y. Ozden Tokatli | E.A. Ozudogru | H. Akdemir | F. Gumusel | A. De Carlo

31. CRYOPRESERVATION OF STRAWBERRY CV. ‘SENGA SENGANA’ BY DROPLET VITRIFICATION

I. Pinker | A. Halmagyi | K. Olbricht | F. Ehrig

32. PRELIMINARY RESULTS OF IN VITRO THERMOTHERAPY OF PEAR CULTIVARS

33. THERMOTHERAPY OF APRICOT CULTIVARS

B. Křižan | E. Ondrušiková

34. THE FUNCTIONAL PROPERTIES OF SWEET CHERRY AS A NEW CRITERION IN A BREEDING PROGRAM

35. EVALUATION OF DRY MATTER IN SOUR CHERRY (PRUNUS CERASUS L.)

C. Grafe | M. Höfer | M. Schuster

36. DEVELOPMENT OF A SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY IN APPLE (MALUS SP.) DURING IN VITRO REGENERATION

M.K. Höhnle | G. Weber

37. MINERAL COMPOSITION OF SOME WALNUT CULTIVARS (JUGLANS REGIA L.) FOR EVALUATION OF IONOME AND IONOMICS UNDER SALT STRESS CONDITION

N. Lotfi | K. Vahdati | B. Kholdebarin | D. Hassani | R. Amiri

38. A REVIEW ON BIOACTIVE COMPOUNDS IN BLACK CURRANTS (RIBES NIGRUM L.) AND THEIR POTENTIAL HEALTH-PROMOTING PROPERTIES

39. EVALUATION OF MEANS TO INCREASE THE CONTENT OF BIOACTIVE PHENOLIC COMPOUNDS IN SOFT FRUITS

M.J. Anttonen | R.O. Karjalainen

40. EFFECT OF HEXANAL TREATMENT ON POSTHARVEST QUALITY OF ‘DARSELECT’ STRAWBERRY (FRAGARIA ×ANANASSA DUCH.) FRUIT

H.Y. Yuan | X.L. Hu | X. Li | Y.H. Li | P. Gopinadhan

41. STUDY ON SOFTENING OF VINAGRILLO (AVERRHOA BILIMBI) FRUITS DURING RIPENING

A. Guadarrama | E. González

42. STERILIZATION OF NON-AUTOCLAVABLE VESSELS AND CULTURE MEDIA BY SODIUM HYPOCHLORITE FOR IN VITRO CULTURE

K. Matsumoto | M.C.F. Coelho | D.C. Monte | J.B. Teixeira

43. THE REACTION OF RASPBERRY AND BLACKBERRY CULTIVARS TO DROUGHT STRESS SIMULATED IN VITRO BY POLYETHYLENE GLYCOL (PEG) 6000

T. Orlikowska | D. Kucharska | M. Horbowicz

44. EFFECT OF SUCROSE LEVEL ON THE PHOTOSYNTHETIC ABILITY OF IN VITRO CULTIVATED APPLE ROOTSTOCK MM 106

L. Nacheva | K. Ivanova | Z. Zlatev

45. EFFECT OF ANTIBIOTICS ON REGENERATION AND ELIMINATION OF BACTERIA DURING GENE TRANSFER IN APPLE

M. Modgil | R. Sharma

46. PRELIMINARY MOLECULAR EVIDENCE THAT QUANTITATIVE POLYMERASE CHAIN REACTION DETECTS CHIMERAS IN TRANSGENIC PLANTS

M. Faize | L. Faize | L. Burgos

47. A PRELIMINARY STUDY ON GENOTYPE-INDEPENDENT AGROBACTERIUM-MEDIATED TRANSFORMATION METHOD TO OBTAIN GENETICALLY ENGINEERED APRICOT PLANTS

48. A HIGH-THROUGHPUT TRANSFORMATION SYSTEM IN PLUM (PRUNUS DOMESTICA L.) USEFUL FOR FUNCTIONAL GENOMICS IN ROSACEAE

C. Petri | K. Webb | CH. Dardick | R. Scorza

49. AGROBACTERIUM-MEDIATED TRANSFORMATION OF A WALNUT CULTIVAR

H. Wang | M.D. Nortes | L. Burgos | N. Alburquerque | J.M. López

50. AGROBACTERIUM-MEDIATED TRANSFORMATION OF OLIVE (OLEA EUROPAEA L.) EMBRYOGENIC CULTURES

R. Torreblanca | E. Palomo-Ríos | S. Cerezo | J.A. Mercado | F. Pliego-Alfaro

51. SYSTEMIC ACQUIRED SILENCING OF A GUSA TRANSGENE IN APPLE

52. SILENCING IN GENETICALLY ENGINEERED PRUNUS DOMESTICA PROVIDES DURABLE AND SAFE RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE)

M. Ravelonandro | R. Scorza

53. DEVELOPMENT OF CISGENIC APPLES WITH DURABLE RESISTANCE TO APPLE SCAB

54. GENES ENCODING FLAVONOID 3′-HYDROXYLASE IN APPLE AND THEIR TAGGED MOLECULAR MARKERS

Y. Han | S.S. Korban

55. USING FUNCTIONAL GENOMICS TO IDENTIFY MOLECULAR MARKERS FOR FIRE BLIGHT RESISTANCE (ERWINIA AMYLOVORA) IN APPLE (MALUS)

56. THE GENOMICS OF FRUIT QUALITY

57. IDENTIFICATION OF CANDIDATE GENES INVOLVED IN PHYLLOXERA RESISTANCE IN GRAPEVINE ROOTSTOCKS

58. QTL AND CANDIDATE GENE MAPPING FOR AROMA COMPOUNDS IN THE APPLE PROGENY ‘DISCOVERY’ × ‘PRIMA’

F. Dunemann | A. Boudichevskaia | C. Grafe | D. Ulrich | W.E. Weber

59. THE POLYMORPHISM OF THE GENES INVOLVED IN ETHYLENE BIOSYNTHESIS AND PERCEPTION IN APPLE

S. Marić | M. Lukić | R.I. Bošković

60. IDENTIFICATION AND MAPPING OF A NEW APPLE SCAB RESISTANCE GENE

61. RESISTANCE GENE ANALOGUES OF HCRVF2 IN APPLE

62. FIRST EVIDENCE OF D-HPLC EFFICIENCY FOR AN AUTOMATED CDNA-AFLP IN THE APPLE SCAB RESISTANCE MODEL

63. ROLE OF SAMS GENE IN FE UPTAKE MECHANISM OF MALUS XIAOJINENSIS

64. PARENT IDENTIFICATION OF HUNGARIAN APPLE CULTIVARS USING SSR MARKERS

I. Király | M. Tóth | A. Pedryc | J. Halász | T. Deák

65. IDENTIFICATION OF APPLE TREE CULTIVARS GROWING IN BELARUS USING SSR-MARKERS

O.Y. Urbanovich | Z.A. Kazlovskaya

66. PRELIMINARY MOLECULAR MARKER MAPS CONSTRUCTED BY USING AN INTERSPECIFIC CROSS BETWEEN PYRUS COMMUNIS AND P. USSURIENSIS

K. Bokszczanin | A.A. Przybyla

67. FUNCTIONAL DIVERSITY FOR RESPONSE TO WATER DEFICIT AMONG DIFFERENT STRAWBERRY GENOTYPES COMPARED TO THEIR GENETIC STRUCTURE AS ASSESSED BY AFLP MARKERS

F. Razavi | M.C. van Labeke | J. de Riek

68. ISOLATION AND CHARACTERIZATION OF LTR RETROTRANSPOSONS FROM STRAWBERRY GENOME

69. MICROSATELLITE MARKERS (SSR) AS A TOOL TO ASSIST IN IDENTIFICATION OF SWEET (PRUNUS AVIUM) AND SOUR CHERRY (PRUNUS CERASUS)

H. Xuan | R. Wang | M. Büchele | O. Möller | W. Hartmann

70. MAPPING PLUM POX VIRUS RESISTANCE IN APRICOT

71. COMPARATIVE GENOMICS OF THE EVERGROWING LOCUS AMONG APRICOT, PEACH AND POPLAR

72. TARGETED ISOLATION OF NONTIR-NBS-LRR RESISTANCE GENE ANALOGS IN APRICOT

A. Gutermuth | A. Pedryc

73. OPTIMIZATION OF THE PREPARATION OF DS CDNA FROM FLOWER BUDS OF APRICOT FOR THE CDNA-AFLP ANALYSIS

J. Cechova | M. Baranek | M. Pidra

74. ALMOND GENETIC LINKAGE MAP AND QUANTITATIVE TRAIT LOCI FOR FLOWERING TIME IN A ‘NONPAREIL’ × ‘LAURANNE’ POPULATION

75. ASSESSMENT OF GENETIC VARIATION IN CITRUS GERMPLASM OF SOUTH OF IRAN USING SSR MARKERS

A. Khadem Nematollahi | K. Vahdati | B. Golein | A. Nabipour | M. Danesh

76. TRANSCRIPTOMIC ANALYSIS OF ETHYLENE-INDUCED TOLERANCE TO NON-CHILLING PEEL PITTING IN CITRUS FRUIT

B. Establés-Ortiz | M.T. Lafuente | L. González-Candelas | J. Forment | J. Gadea

77. A MAPPING APPROACH TO DEFINE THE GENETIC STRUCTURE OF THE SEX-DETERMINING LOCUS IN THE DIOECIOUS SPECIES ACTINIDIA CHINENSIS

78. TRANSFERABILITY OF MALUS × DOMESTICA MICROSATELLITE MARKERS TO OTHER SPECIES AND GENERA OF THE MALOIDEAE SUBFAMILY

L. Vanwynsberghe | L. Decq | J. Keulemans

79. AFLP-DERIVED METHODS AS A TOOL FOR STUDY OF GENOMIC, EPIGENOMIC AND TRANSCRIPTOMIC CHANGES IN STRESSED GRAPEVINE PLANTS

M. Baranek | B. Křižan | J. Raddová | E. Ondrušíková | M. Pidra

80. CLONAL SELECTION WITHIN GRAPEVINE ‘ASKARI’ BY MOLECULAR MARKERS

81. THE ANTHOCYANIN AND FLAVONOL PROFILE IN VITIS VINIFERA INTRASPECIFIC HYBRIDS

A. Hernández Jiménez | J.M. López-Roca | E. Gómez Plaza | R. Gil-Muñoz | A. Martinez Cutillas

82. MOLECULAR CHARACTERIZATION AND ANALYSIS OF GEOGRAPHICAL DIFFERENTIATION OF INDIAN MANGO (MANGIFERA INDICA L.) GERMPLASM

S. Singh | K.V. Bhat

83. ASSESSING THE USE OF HETEROLOGOUS OLIGONUCLEOTIDE MICROARRAYS FOR TRANSCRIPTOMICS IN A NON-MODEL SPECIES; APPLICATION TO THE STUDY OF DROUGHT STRESS IN MUSA

84. GENETIC DIVERSITY STUDY IN ANNONA SQUAMOSA BY MORPHOLOGICAL, BIOCHEMICAL AND RAPD MARKERS

S.G. Bharad | P.L. Kulwal | S.A. Bagal

85. CONSTRUCTION OF A RELIABLE PCR MARKER FOR SELECTING POLLINATION CONSTANT AND NON-ASTRINGENT (PCNA) TYPE OFFSPRING AMONG BREEDING POPULATION OF PERSIMMON (DIOSPYROS KAKI THUNB.)

K. Yonemori | T. Akagi | S. Kanzaki

86. RNAi-SILENCING OF MdTFL1 INDUCES EARLY FLOWERING IN APPLE

87. CHARACTERIZATION OF TRANSGENIC APPLES EXPRESSING THE HrpN GENE

88. ECTOPIC EXPRESSION OF A SPINACH SOD GENE IN YOUNG APPLE TREES ENHANCES ABIOTIC STRESS RESISTANCE

T.S. Artlip | M.E. Wisniewski | D. Macarisin | J.L. Norelli

89. TOWARDS THE PRODUCTION OF STRESS TOLERANT GRAPEVINE CULTIVARS

90. CLONING AND CHARACTERIZATION OF VvChiF III GENE FROM VITIS VINIFERA CV. ‘FLAME SEEDLESS’

A. Ebadi | S.A. Salami | M.R. Naghavi | I.B. Dry

91. THE DEVELOPMENT OF GENETIC RESISTANCE TO PLUM POX VIRUS IN TRANSGENIC NICOTIANA BENTHAMIANA AND PRUNUS DOMESTICA

92. STILBENE SYNTHASE GENE TRANSFER RESULTED IN DOWN REGULATION OF ENDOGENOUS CHALCONE SYNTHASE IN STRAWBERRY (FRAGARIA × ANANASSA) AND LED TO THE IDENTIFICATION OF NOVEL PHENYLPROPANOID GLUCOSIDES

93. TRANSCRIPTOMIC AND PROTEOMIC RESPONSE OF FRUIT TREES TO ABIOTIC STRESS

94. PLANT SIZE CONTROL IN APPLE THROUGH GENETIC ENGINEERING

L.H. Zhu | X.Y. Li | L. Kāle | M. Welander

95. CLONING AND PRELIMINARY CHARACTERISATION OF THE PROMOTER REGION OF THE 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE GENE

S.M. Granozio | S. Tartarini | P. Negri | S. Sansavini

96. THE SWITCH TO FLOWERING: GENES INVOLVED IN FLORAL INDUCTION OF THE APPLE CULTIVAR ‘PINOVA’ AND THE ROLE OF THE FLOWERING GENE MdFT

97. FRUIT SET REGULATING GENES ARE DIFFERENTLY EXPRESSED AFTER CROSS-POLLINATION, SELF-POLLINATION AND TREATMENT WITH PLANT GROWTH REGULATORS IN MALUS × DOMESTICA CV. ‘JONAGOLD’

98. ENVIRONMENTAL RISK ASSESSMENT OF GM PLANTS AT THE EUROPEAN LEVEL

J. Schiemann

99. FIRST RESULTS ON THE EFFECT OF INCREASED CHITINASE EXPRESSION IN TRANSGENIC APPLE TREES ON MYCORRHIZATION WITH GLOMUS INTRARADICES AND G. MOSSEAE