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Articles

A HIGH-THROUGHPUT TRANSFORMATION SYSTEM IN PLUM (PRUNUS DOMESTICA L.) USEFUL FOR FUNCTIONAL GENOMICS IN ROSACEAE

Article number
839_48
Pages
375 – 379
Language
English
Abstract
We have developed an improved Agrobacterium-mediated protocol in plum (Prunus domestica L.) through the addition of 2,4-D to the regeneration media.
This method has increased the regeneration efficiency of independent transgenic plants up to 10 fold over previous reports.
DNA blot analysis of putative transgenic shoots revealed transformation efficiencies of up to 42% with an average of 25% over all trials.
The method as described produces self-rooted transgenic plants in approximately 6 months.
The high transformation rates coupled with the rapid plant establishment methodology makes it possible to utilize plum transformation both for the introduction of agronomically useful genes into this species, and as a model plant for functional genomics studies in Prunus spp., rosaceaous species, and woody plants in general.

Publication
I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008
Authors
C. Petri, K. Webb, CH. Dardick, R. Scorza
Keywords
Agrobacterium tumefaciens, gene function, genetic transformation, Prunus spp.
Full text
https://doi.org/10.17660/ActaHortic.2009.839.48
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